| 黄瓜根际土壤细菌群落的16S rDNA-PCR-DGGE分析 |
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| 引用本文： | 赵柏霞,闫建芳,刘 秋,于基成,赵秀香.黄瓜根际土壤细菌群落的16S rDNA-PCR-DGGE分析[J].中国蔬菜,2015,1(12):33-37. |
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| 作者姓名： | 赵柏霞 闫建芳 刘 秋 于基成 赵秀香 |
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| 作者单位： | （;1. 大连市农业科学研究院，辽宁大连 116036；;2. 沈阳农业大学植物保护学院，辽宁沈阳 110161；;3. 大连民族大学生命科学学院，辽宁大连 116600） |
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| 基金项目： | 国家自然科学基金项目（31070005，21276047，31270057，31101477），中央高校基本科研业务费自主基金青年项目（DC13010309，DC12010103） |
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| 摘 要： | 采用PCR-DGGE技术分析了接种枯萎病菌后黄瓜根际土壤细菌群落的动态变化。对黄瓜种植前、接菌前、接菌后发病、接菌后未发病以及接种清水(对照)共5份土壤样品直接提取其总DNA,用F338-GC/R518引物扩增16Sr DNA基因的V3可变区,结合DGGE电泳条带分析样品的细菌群落组成及变化趋势。结果显示,接种枯萎病菌后,会引起黄瓜根际土壤中细菌数量及种类发生较大的变化,其中,接种枯萎病菌后Rhodococcus phenolicus(E1)、Clostridiumsp.(E3、E6)以及3种未培养细菌(E2、E4、E5)数量增加,另外2种未培养细菌(A1、A2)数量减少。
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| 关 键 词： | 根际微生物 PCR-DGGE 枯萎病菌 细菌群落 |
Analysis of the Bacterial Communities in Rhizosphere Soil of Cucumber Using 16S rDNA -PCR- DGGE |
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| Institution: | （;1.Dalian City Academy of Agricultural Sciences， Dalian 116036， Liaoning，China；;2.College of Plant Protection，Shenyang Agricultural University， Shenyang 110161， Liaoning，China；;3. College of Life Science， Dalian Nationalities University， Dalian 116600， Liaoning，China） |
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| Abstract: | 16S rDNA-PCR-DGGE was used to analyze the bacterial community dynamic changes in rhizosphere soil infected by cucumber Fusarium wilt. There are 5 rhizosphere soil samples， including pre-planting soil， pre-inoculation soil， diseased cucumber and healthy cucumber after inoculation， not inoculated as the control. Total DNA was directly extracted and amplified with F338-GC and R518 primers targeting the 16S rDNA
V3. The amplified were analyzed by DGGE analysis. The result revealed that there was a high diversity of bacterial community compositions among different tested soil samples. Fusarium caused a great bacterial species and number changes in rhizosphere soil， of which Rhodococcus phenolicus， Clostridium sp. and 3 uncultured bacteria increased， another 2 uncultured bacteria reduced after inoculation. |
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| Keywords: | Rhizosphere microbe PCR-DGGE Fusarium oxysporum Bacterial communities |
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