Diagnosis and Treatment of Mycoplasma bovis Pneumonia with Bovine Capsular Serotype A Pasteurella multocida Infection in Calf |
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Authors: | LAI Jin-lun KOU Ming-ming LIU Yu-hui ZHU Xi-fang LIU Jing JIANG Peng CHEN Ying-yu HU Chang-min GUO Ai-zhen |
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Institution: | 1. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;2. State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China |
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Abstract: | The assay was aimed to clarify the reasons why pneumonia and arthritis had happened in a dairy farm,and provide effective control and treatment.A newborn calf was autopsied and the samples were collected.Mycoplasma bovis (M.bovis) and other pathogens were isolated and cultured,and PCR identification and drug sensitivity test were performed.Bovine viral diarrhea virus (BVDV),foot-and-mouth disease virus (FMDV) and infectious bovine rhinotracheitis virus (IBRV) were detected by PCR.Pathological slices from calf lung tissue were made to evaluate pathological changes.M.bovis and bovine capsular serotype A Pasteurella multocida (Pm A) were isolated,respectively.There were negative results for BVDV,FMDV and IBRV detection.Pathological slices of lung tissue showed damaged visible alveolar structure,bleeding and a large number of inflammatory cells infiltration.Drug sensitivity test results showed that M.bovis and Pm A were sensitive to tylosin and cefazolin,respectively,but resistance to penicillin,gentamicin,clindamycin and ampicillin.It was confirmed that M.bovis pneumonia with Pm A infection had happened.The disease in the farm was controlled by using tylosin and cefazolin at the same time.Furthermore,symptomatic treatment and standardized management should be carried. |
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Keywords: | calf Mycoplasma bovis pneumonia bovine capsular serotype A Pasteurella multocida diagnosis treatment |
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