Injury effect of recombinant soluble human CD40 ligand on human umbilical vein endothelial cells |
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| Authors: | LIU Hong-li CHEN Meng WANG Hong-tao WU Yi-ling |
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| Institution: | 1. Nanjing University of Chinese Medicine, Nanjing 210023, China;
2. Hebei Yiling Medical Research Institute, Shijiazhuang 050035, China;
3. Key Research Centre of State Administration of Traditional Chinese Medicine (Collateral Disease of Cardiovasculature), Shijiazhuang 050035, China;
4. Key Laboratory of Collateral Disease of Hebei Province, Shijiazhuang 050035, China |
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| Abstract: | AIM: To investigate the damage in human umbilical vein endothelial cells (HUVECs) induced by recombinant soluble human CD40 ligand (rshCD40L). METHODS: The cultured HUVECs were treated with rshCD40L for 12 h. The survival activity of the HUVECs was observed by MTS assay. The expression of E-selectin, intercellular adhesion molecule (ICAM)-1, tissue factor (TF) and tissue factor pathway inhibitor (TFPI) was measured by ELISA. The activity of superoxide dismutase (SOD) and the level of malondialdehyde (MDA) were detected by the methods of thibabituric acid (TBA). RESULTS: Compared with normal group, different concentrations of rshCD40L (0.5, 1, 2, 3 mg/L) had no obvious effect on the survival activity of the HUVECs (P>0.05). rshCD40L at concentration of 0.5 mg/L promoted the secretion of E-selectin, sICAM-1, TF and TFPI in the HUVECs (P<0.01). rshCD40L at concentration of 0.5 mg/L also increased MDA content and reduced the activity of SOD in the HUVECs (P<0.05). CONCLUSION: 0.5~3mg/L rshCD40L has no obvious effect on endothelial cell survival, but already causes endothelial dysfunction by increasing endothelial inflammation and exogenous coagulation reaction, inducing lipid peroxides injury and reducing antioxidant capacity. |
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| Keywords: | Recombinant soluble human CD40 ligand Human umbilical vein endothelial cells Atherosclerosis |
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