The Correlation Analysis between Chinese Merino Sheep OLA-DQB1 Gene Exon 2 Genetic Polymorphism and Brucellosis Susceptibility |
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| Authors: | WANG Wen-wen XU Wan-yun HU Meng-wei LI Jian-hua LIU Peng-tao GAO Jian-feng |
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| Institution: | College of Life Science, Shihezi University, Shihezi 832003, China |
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| Abstract: | The single nucleotide polymorphisms (SNPs) of ovine lymphocyte antigen DQB1 (OLA-DQB1) gene exon 2 was amplified by PCR-SSCP method from 148 healthy and 60 infected with Brucella Chinese Merino sheep and then PCR products of different alleles were sequenced to determine the polymorphism loci of the gene.The differences in gene frequency and genotype frequency of each SNP loci were analyzed statistically to analyze its correlation with brucellosis susceptibility.The sequencing result showed that 43 SNPs were detected in 270 bp DNA sequence,the gene frequencies of G196A allele had extremely significant difference in case and control samples (P< 0.01),and its genotype frequencies presented significant difference (P< 0.05).Similarly,C211T allele was significantly different in case and control samples (P< 0.05).The results showed that the polymorphism of OLA-DQB1 gene exon 2 might be a significant association gene with brucellosis susceptibility. |
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| Keywords: | PCR-SSCP Chinese Merino sheep OLA-DQB1 gene single nucleotide polymorphisms (SNPs) brucellosis susceptibility |
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