Effects of iPSC-MSCs on mitochondria of PC12 cells injured by CoCl2 |
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| Authors: | YANG Yan LI Hui SUN Zhan-peng HU Chun-lin JING Xiao-li WEI Hong-yan LIAO Xiao-xing LI Xin |
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| Institution: | Department of Emergency, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China |
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| Abstract: | AIM: To investigate the effects of induced pluripotent stem cells-derived mesenchymal stem cells (iPSC-MSCs) on cobalt chloride (CoCl2)-induced injuries of PC12 cells and its possible mechanism. METHODS: PC12 cells were exposed to CoCl2 to set up a chemical-induced cellular injury model and were cocultured with iPSC-MSCs. The cell viability was tested by CCK-8 assay. The apoptosis was measured by flow cytometry using Annexin V/PI staining. The mitochondrial membrane potential (MMP) was analyzed by flow cytometry using JC-1 staining. Immunofluorescence was employed to observe mitochondrial transfer from iPSC-MSCs to PC12 cells. RESULTS: Apoptosis of PC12 cells was increased and MMP of PC12 cells was decreased after exposed to CoCl2 at concentration of 400 μmol/L for 24 h. Coculture of PC12 cells with iPSC-MSCs reduced the apoptosis and recovered the MMP of the PC12 cells. Tunneling nanotubes were formed between iPSC-MSCs and PC12 cells, through which the iPSC-MSCs transferred the mitochondria to the PC12 cells. CONCLUSION: iPSC-MSCs protect PC12 cells from CoCl2-induced injuries, which may be associated with the mitochondrial transfer from iPSC-MSCs to PC12 cells. |
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| Keywords: | Cobalt chloride PC12 cells Apoptosis Induced pluripotent stem cells Mitochondria |
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