Ultrastructural impairment of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice |
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| Authors: | ZHANG Xu LIU Ming-ming CHENG Ming XU Jin WANG Shu-he WANG Hui REN Ya-li XIU Rui-juan WANG Su-xia |
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| Institution: | 1. Laboratory of Electron Microscopy, Ultrastructural Pathology Center, Peking University First Hospital, Beijing 100034, China;
2. Institute of Microcirculation, Key Laboratory of Microcirculation, Ministry of Health, CAMS & PUMC, Beijing 100005, China |
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| Abstract: | AIM: To investigate the ultrastructural changes of islet microvascular endothelial cells in STZ-induced type 1 diabetic mice. METHODS: BALB/c mice were randomly divided into diabetic group and control group. The expression of insulin and platelet-endothelial cell adhesion molecule-1 (CD31) in islet microvessels was detected by immunohistochemical staining. The ultrastructural changes of islet β cells and islet microvessels were observed under transmission electron microscope. RESULTS: Compared with control group, the number of islet β cells, ratio of β cells/α cells, average number of secretory granules in β cells and insulin expression area per islet in diabetic group were significantly decreased (P<0.01). Besides, diabetic group had fewer microvessels with lower expression of CD31 (P<0.01). Mitochondria in islet microvascular endothelial cells and pericytes in diabetic group were swelling. The basement membrane of islet microvessels became thicker in diabetic group (P<0.01). CONCLUSION: Islet microvascular endothelial cells were impaired in type 1 diabetic mice. |
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| Keywords: | Islet microvascular endothelial cells Ultrastructure Type 1 diabetes Islet microcirculation |
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