| 鹅掌楸属种及杂种的SSR分子鉴定 |
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| 引用本文： | 张红莲,李火根,胥猛,冯源恒.鹅掌楸属种及杂种的SSR分子鉴定[J].林业科学,2010,46(1). |
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| 作者姓名： | 张红莲 李火根 胥猛 冯源恒 |
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| 作者单位： | 南京林业大学林木遗传与生物技术省部共建教育部重点实验室,南京,210037 |
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| 基金项目： | 国家自然科学基金项目(30771748);;国家“十一五”科技支撑课题(2006BAD01A16);;江苏省自然科学基金项目(BK2006211) |
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| 摘 要： | 以不同种源的鹅掌楸、北美鹅掌楸和不同组合的杂交鹅掌楸为试验材料,对176对SSR引物进行初筛、复筛,最后筛选出1对物种特异性SSR引物。该引物在鹅掌楸群体中特异性扩增出190bp的产物,在北美鹅掌楸群体中特异性扩增出180bp的产物,在杂交鹅掌楸群体中特异性扩增出180bp和190bp2种产物。该引物对鹅掌楸种间鉴别准确率达100%。
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| 关 键 词： | 鹅掌楸 北美鹅掌楸 杂交鹅掌楸 SSR分子标记 特异性鉴定 |
Identification of Liriodendron tulipifera,Liriodendron chinense and Hybrid Liriodendron Using Species-Specific SSR Markers |
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| Zhang Honglian,Li Huogen,Xu Meng,Feng Yuanheng.Identification of Liriodendron tulipifera,Liriodendron chinense and Hybrid Liriodendron Using Species-Specific SSR Markers[J].Scientia Silvae Sinicae,2010,46(1). |
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| Authors: | Zhang Honglian Li Huogen Xu Meng Feng Yuanheng |
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| Institution: | Key Laboratory of Genetics & Biotechnology of Ministry of Education Nanjing Forestry University Nanjing 210037 |
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| Abstract: | This paper reported that species-specific SSR markers were used to identify Liriodendron tulipifera,L.chinense and hybrid Liriodendron.Overall,90 genotypes were taken as DNA amplification templates,of which 30 came from 20 provenances of L.chinense,30 from 5 provenances of L.tulipifera,and 30 from 30 mating combinations of hybrid Liriodendron.Upon two round amplifying and screening,one species-specific pair primers for Liriodendron were screened from 176 pair primers.With the pair primers,a 190 bp fragment ... |
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| Keywords: | Liriodendron tulipifera Liriodendron chinense hybrid Liriodendron SSR marker specific identification |
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