Seminal plasma lactoferrin but not transferrin reflects gonadal function in dogs |
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| Authors: | Kikuchi Motohiro Mizoroki Shohei Kubo Takashi Ohiwa Yoshiko Kubota Miho Yamada Naoki Orino Koichi Ohnami Yohji Watanabe Kiyotaka |
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| Institution: | Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori, Japan. |
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| Abstract: | Lactoferrin purified from canine seminal plasma by a three-step chromatography procedure had a molecular mass of 75.2 kDa and cross-reacted with antiserum to equine seminal plasma lactoferrin. Seminal plasma lactoferrin concentrations were determined by a competitive enzyme-linked immunosorbent assay (ELISA) by using rabbit anti-equine lactoferrin antibody and alkaline phosphatase-labeled goat anti-rabbit IgG antibody in 14 normal dogs and found to range from 12 to 197 micro g/ml, with a mean value of 77 +/- 59 micro g/ml (the mean +/- SD). Seminal plasma transferrin concentrations were determined by a sandwich ELISA with goat antibody to canine serum transferrin and alkaline phosphatase-conjugated goat anti-canine transferrin antibody and found to range from 0.32 to 12.6 micro g/m l, with a mean value of 2.44 +/- 3.25 micro g/m l. The lactoferrin concentration significantly correlated with the sperm concentration (r=0.7025, P<0.01), but there was no significant correlation between the seminal plasma transferrin concentration and sperm density. These results indicate that seminal plasma lactoferrin, but not transferrin, reflects gonadal function. |
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