| 两种大眼蟹线粒体12S rRNA基因序列的比较研究 |
| |
| 引用本文： | 徐敬明.两种大眼蟹线粒体12S rRNA基因序列的比较研究[J].水产科学,2009,28(12). |
| |
| 作者姓名： | 徐敬明 |
| |
| 作者单位： | 重庆文理学院,生命科学与技术学院,重庆,402168 |
| |
| 基金项目： | 重庆文理学院引进人才专项 |
| |
| 摘 要： | 测定了宽身大眼蟹和日本大眼蟹线粒体12S rRNA基因部分片段的序列,前者为577 bp, 后者为572 bp.二者的核苷酸序列A、T、G、C的含量相似,分别为39.0%、35.7%、16.5%、8.8%;39.3%、 36.2%、 15.8%、8.7%;不包括11处插入/缺失位点,两序列间有75个变异位点,核苷酸差异率为13.18%,其中转换42个、颠换33个, 转换与颠换比约为1.3.对国内外4种大眼蟹长度为331 bp的12S rRNA基因同源序列进行分析,A+T的平均含量为76.6%,明显高于G+C的平均含量,且存有变异位点52个,简约信息位点25个.系统发生树的拓扑结构显示,所有的大眼蟹聚为一支,支持大眼蟹类为一单系.
|
| 关 键 词： | 宽身大眼蟹 日本大眼蟹 序列 系统发生 |
Sequence Comparison of Mitochondrial 12S rRNA Genes between Two Crab Species,Macrophthalmid dilatatum and M. japonicus |
| |
| XU Jing-ming.Sequence Comparison of Mitochondrial 12S rRNA Genes between Two Crab Species,Macrophthalmid dilatatum and M. japonicus[J].Fisheries Science,2009,28(12). |
| |
| Authors: | XU Jing-ming |
| |
| Institution: | XU Jing-ming(College of Life Science , Technology,Chongqing University of Arts , Sciences,Chongqing 402168,China) |
| |
| Abstract: | Partial sequences of the mitochondrial 12S rRNA gene were determined for two species crabs,Macrophthalmus dilatatum and M.japonicus.The results showed that the 12S rRNA gene had 577 bp for M.dilatatum and 572 bp for M.japonicus with similar contents of A(39.0 % for M.dilatatum;39.3 % for M.japonicus),T(35.7 %,36.2 %),G(16.5 %,15.8 %)and C(8.8 %,8.7 %).There were 75 different sites(except 11 deletion/insertion sites)between the two species,including 42 transition sites and 33 transversion sites.It was found ... |
| |
| Keywords: | 12S rRNA Macrophthalmus dilatatum M japonicus 12S rRNA sequence phylogeny |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
