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Differentiation of Australian isolates of Mycobacterium paratuberculosis using pulsed-field gel electrophoresis |
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| Authors: | MM FEIZABADI ID ROBERTSON A HOPE DV COUSINS DJ HAMPSON |
| Institution: | Division of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150;Victorian Institute of Animal Science, Mickleham Road, Attwood, Victoria 3049;Australian Reference Laboratory for Bovine Tuberculosis, Agriculture Western Australia, South Perth, Western Australia 6151 |
| Abstract: | Objectives To examine strain variation amongst Australian isolates of Mycobacterium paratuberculosis . Design Pulsed field gel electrophoresis was optimised for differentiation of M paratuberculosis strains, and this typing technique was then applied to a collection of Australian isolates. Procedure DNAs from 35 Australian isolates of M para-tuberculosis and a UK reference strain were digested with one or other of three restriction endonucleases. The banding patterns obtained after pulsed field gel electrophoresis of the DNA fragments were compared. Results The Australian isolates were divided into two groups on the basis of their DNA banding pattern. Both were different from the UK reference strain. Seven isolates from cattle in Victoria and the Northern Territory had the same pattern as five isolates from alpacas in Victoria and Western Australia. Another 20 isolates from cattle in Victoria, Western Australia and the Northern Territory had the same pattern as isolates from two sheep and a goat in New South Wales. Conclusion Pulsed field gel electrophoresis was a useful tool for strain typing of M paratuberculosis , and could be used to study the transmission of strains in Australia. |
| Keywords: | Mycobacterium paratuberculosis strain typing PFGE |