Genetic Diversity of Japanese Melon Cultivars (<Emphasis Type="Italic">Cucumis melo</Emphasis> L.) as Assessed by Random Amplified Polymorphic DNA and Simple Sequence Repeat Markers |
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| Authors: | Eijiro?Nakata Email author" target="_blank">Jack?E?StaubEmail author Ana?I?López-Sesé Nurit?Katzir |
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| Institution: | (1) Sakata Seed, 1743-2, Yoshioda, Kakegawa-Shi, Shizuoka-Ken 436-0115, Japan;(2) US Department of Agriculture, Agricultural Research Service, Vegetable Crops Research Unit, Department of Horticulture, University of Wisconsin Madison, 1575 Linden Dr., Madison, WI 53706, USA;(3) Newe Ya’ar Research Center, Agricultural Research Organization, Ramat Yishay, 30095, Israel |
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| Abstract: | The genetic diversity among 67 melon (C. melo L.) cultivars from five Japanese seed companies was assessed using 25 10-mer
RAPD primers (56 bands) and nine SSR (36 alleles) markers. These cultivars belong to three horticultural varieties (synom.
Groups) spanning eight melon market classes: Group Cantalupensis (market classes Earl’s, House, Galia, Charentais, and Ogen),
Group Inodorus Honeydew and Casaba melons (market classes Amarillo, Piel de Sapo, Rochet, Negro, Crenshaw, and Tendral)],
and Group Conomon (market class Oriental). Genetic variation among these cultivars was compared to variation in a reference
array (RA) consisting of 34 selected melon accessions from previous studies. Cluster analysis resulted in 11 of 15 Japanese
Oriental accessions forming a group with South African RA accessions. The remaining Group Conomon Japanese accessions grouped
either with Casaba or with Honeydew cultivars. Japanese Group Conomon accessions and South African RA accessions formed a
genetic group that was distinct from all other accessions studied, and suggests either an Asiatic origin for the South African
melon germplasm examined or an independent domestication involving similar ancestors. The majority of Japanese House and Earl
market class accessions shared genetic affinities, and were genetically different from the Japanese Group Inodorus accessions
examined. These Japanese accessions were most similar to Casaba RA accessions. Japanese Galia accessions were similar to either
House and Earl’s market classes or to Galia, Ogen, Casaba, and Honeydew RA accessions. Genetic differences exist between melon
types that were domesticated from wild, ‘free-living’ subspecies agrestis and from melo.
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| Keywords: | Diversity analysis Genetic distance Germplasm management RAPD SSR |
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