| 猪睾丸中脂联素受体与LHR、CYP11A1、StAR基因表达的发育变化及其相关性研究 |
| |
| 引用本文： | 邵康,周杰,吴小雪,舒宝屏,罗联辉,盛晟,张佳,李维新,殷宗俊.猪睾丸中脂联素受体与LHR、CYP11A1、StAR基因表达的发育变化及其相关性研究[J].畜牧兽医学报,2011,42(12). |
| |
| 作者姓名： | 邵康 周杰 吴小雪 舒宝屏 罗联辉 盛晟 张佳 李维新 殷宗俊 |
| |
| 作者单位： | 1. 安徽农业大学动物科技学院,合肥,230036
2. 黟县畜牧兽医局,黟县,245500 |
| |
| 基金项目： | 国家自然科学基金项目(30771581); 安徽省“十一五”科技攻关计划重大科技专项(08010301079) |
| |
| 摘 要： | 旨在了解皖南花猪睾丸组织脂联素受体mRNA与睾酮合成相关因子mRNA表达的发育性变化及其相关性,探讨脂联素与睾酮生成的关系.本研究用荧光实时定量PCR法检测0、30、45、90和180 d等不同日龄(各日龄样本数5头)皖南花猪睾丸组织脂联素受体(AdpR1、AdpR2)和LHR、CYP11A1、StAR mRNA的表达水平.结果,AdpR1、CYP11 A1和StAR mRNA的表达有极显著的发育性变化(P＜0.01),AdpR1 mRNA的表达先增加后降低,45 d达到最大值；CYP11A1和StAR mRNA的表达先升高后降低再升高,45 d达到最大表达量.LHR mRNA的表达也有显著的发育性变化(P＜0.05),整体表现为先升高后降低.AdpR1与CYP11A1 mRNA的表达量显著相关(r=0.587,P＜0.05)；LHR与CYP11A1、StAR mRNA的表达量分别显著相关(r=0.528,P＜0.05；r=0.552,P＜0.05)；CYP11 A1与StAR mRNA表达量极显著相关(r=0.709,P＜0.01).AdpR1mRNA在睾丸组织的高表达提示脂联素可能通过AdpR1介导对睾丸的调节作用,其发育性变化和CYP11A1 mRNA呈正相关,提示脂联素对睾酮的生成有一定的促进作用.
|
| 关 键 词： | 皖南花猪 脂联素受体 睾酮合成相关因子 发育性变化 |
The Developmental Patterns and Correlation of Adiponectin Receptors,LHR,CYP11A1 and StAR mRNA Expression in Testis of Wannan Hua Pigs |
| |
| SHAO Kang
,
ZHOU Jie
,
WU Xiao-xue
,
SHU Bao-ping
,
LUO Lian-hui
,
SHENG Sheng
,
ZHANG Jia
,
LI Wei-xin
,
YIN Zong-jun.The Developmental Patterns and Correlation of Adiponectin Receptors,LHR,CYP11A1 and StAR mRNA Expression in Testis of Wannan Hua Pigs[J].Acta Veterinaria et Zootechnica Sinica,2011,42(12). |
| |
| Authors: | SHAO Kang ZHOU Jie WU Xiao-xue SHU Bao-ping LUO Lian-hui SHENG Sheng ZHANG Jia LI Wei-xin YIN Zong-jun |
| |
| Institution: | SHAO Kang1,ZHOU Jie1,WU Xiao-xue1,SHU Bao-ping2,LUO Lian-hui2,SHENG Sheng1,ZHANG Jia1,LI Wei-xin1,YIN Zong-jun1 (1.College of Animal Science and Technology,Anhui Agricultural University,Hefei 230036,China,2.Animal Husbandry and Veterinary Medicine Bureau of Yi County,Yixian 245500,China) |
| |
| Abstract: | The aims of this study were to observe the developmental patterns and the correlation of adiponectin receptors and testosterone synthesis-related factors mRNA expressions in testis of Wannan Hua pig.To investigate the relationship of adiponectin with the generation of testosterone.Five Wannan Hua boars were sampled at birth,30,45,90,and 180 days of age,respectively.The expression of AdpR1,AdpR2,LHR,CYP11A1 and StAR mRNA in testis were detected by real-time fluorescence quantitative RT-PCR.The expression of ... |
| |
| Keywords: | Wannan Hua pig adiponectin receptor testosterone synthesis-related factor developmental patterns |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
