| 邓恩桉种子组织培养的研究 |
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| 引用本文： | 宋建英.邓恩桉种子组织培养的研究[J].中南林业科技大学学报,2008,28(6). |
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| 作者姓名： | 宋建英 |
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| 作者单位： | 福建林业职业技术学院 |
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| 基金项目： | 福建省林业厅科技推广项目 |
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| 摘 要： | 以邓恩桉种子为外植体,探讨用最少种子快速繁殖最多幼苗的方法,结果表明:MS培养基是较适合邓恩桉种子萌芽和生长的基本培养基;诱导种子直接脱分化成愈伤组织的较佳培养基配方为MS KT1.0 mg/L 2,4-D2.0 mg/L 半胱氨酸30 mg/L;以邓恩桉实生苗的芽来繁芽的较理想培养基配方为H BA2.0 mg/L IAA0.2 mg/L;邓恩桉实生苗的根诱导愈伤组织的较佳培养基配方为MS 6-BA2.0 mg/L NAA0.2 mg/L;诱导邓恩桉下胚轴分化芽较佳培养基配方为B5 6-BA2.0 mg/L 2,4-D0.2 mg/L;诱导邓恩桉下胚轴脱分化为胚性愈伤组织的较佳培养基配方为B5 Ad2.0 mg/L IAA0.2 mg/L.
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| 关 键 词： | 生物技术 邓恩桉 种子 芽苗 愈伤组织 培养基 |
Tissue Culture of Eucalyptus dunnii Seeds |
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| SONG Jian-ying.Tissue Culture of Eucalyptus dunnii Seeds[J].Journal of Central South Forestry University,2008,28(6). |
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| Authors: | SONG Jian-ying |
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| Abstract: | This paper presents a study of Eucalyptus dunnii tissue culture,using its seeds as explants.The results reveal that MS medium is a suitable basic medium for its seeds to germinate and grow;that MS KT1.0 mg/L 2,4-D2.0 mg/L Homocysteine30 mg/L is a good medium to induce its seeds to dedifferentiate directly into callus;that H 6-BA2.0 mg/L IAA0.2 mg/L is a good medium to make its bud on the seedling reproduce more buds;that MS 6-BA2.0 mg/L NAA0.2 mg/L is a better medium to induce its root on the seedling into callus;that B5 6-BA2.0 mg/L 2,4-D0.2 mg/L can induce its under-hypocotyl to differentiate into buds; and that B5 Ad2.0 mg/L IAA0.2 mg/L can induce its under-hypocotyl into callus to generate normal buds. |
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| Keywords: | biotechnology Eucalyptus dunnii seeds buds callus medium |
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