| 人心果AFLP反应体系的建立与优化 |
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| 引用本文： | 文亚峰,谢碧霞,何钢.人心果AFLP反应体系的建立与优化[J].经济林研究,2008,26(2). |
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| 作者姓名： | 文亚峰 谢碧霞 何钢 |
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| 作者单位： | 1. 中南林业科技大学资源与环境学院,湖南,长沙,410004
2. 中南林业科技大学生命科学与技术学院,湖南,长沙,410004 |
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| 基金项目： | 国家林业局"948"国际引进项目"人心果优良品种及其培育技术引进" |
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| 摘 要： | 为给人心果分子生物学研究奠定基础,以人心果嫩叶为材料,采用改良CTAB法提取到高质量的总DNA,可用于AFLP实验分析。通过优化酶切—连接反应、预扩增和扩增反应过程中的关键因素,建立了适合人心果的AFLP实验体系和反应条件,得到了带型清晰、多态性丰富的AFLP指纹图谱。研究结果对人心果遗传多样性分析及其分子标记辅助育种具有重要意义。
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| 关 键 词： | 人心果 AFLP 反应体系 PCR |
Establishment and Optimization of AFLP System in Manilkara zapotae |
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| WEN Ya-feng,XIE Bi-xia,HE Gang.Establishment and Optimization of AFLP System in Manilkara zapotae[J].Economic Forest Researches,2008,26(2). |
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| Authors: | WEN Ya-feng XIE Bi-xia HE Gang |
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| Abstract: | In order to provide a basis for molecular biology of Manilkara zapota,improved CTAB method was used to extract high quality genomic DNA from leaves of M. zapota,which could be used in amplified fragment-length polymorphism(AFLP) analysis.PCR reaction system and conditions suitable for M.zapota were established,through optimization of some key factors affecting restriction-ligation reaction,pre-amplification and selective amplification reactions,and clear polymorphic AFLP fingerprints were obtained.The results were important for analysis of genetic diversity and molecular marker assisted breeding in M.zapota. |
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| Keywords: | Manilkara zapota AFLP reaction system PCR |
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