| 用DNA限制性片段长度多态性鉴定旋毛虫各隔离种 |
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| 引用本文： | 宋铭忻,张桂红,路义鑫,蒋成玉.用DNA限制性片段长度多态性鉴定旋毛虫各隔离种[J].中国兽医寄生虫病,2001,9(1):5-8. |
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| 作者姓名： | 宋铭忻 张桂红 路义鑫 蒋成玉 |
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| 作者单位： | 1. 东北农业大学动物医学院哈尔滨150030
2. 黑河出入境检验检疫局黑河164300 |
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| 基金项目： | 黑龙江省"九五”攻关课题(G96B3-4)和黑龙江省自然科学基金(C094008) |
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| 摘 要： | 以旋毛虫国际标准虫种：旋毛形线虫（Trichinella spirlais）和本地长形线虫(Trichinella nativa）作对照，应用DNA限制性片段长度多态性（PELP）技术对黑龙江省猪、犬旋毛虫进行虫种鉴定。结果显示：猪旋毛虫和旋毛形线虫酶切图谱相同；犬旋毛虫和本地毛形线虫酶谱一致。结果提示，黑龙江猪旋毛虫为旋毛形线虫，犬旋毛虫为本地毛形线虫。
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| 关 键 词： | 旋毛虫 限制性片段长度多态性 虫种 隔离种 鉴定 |
| 文章编号： | 1005-0868(2001)01-0005-04 |
| 修稿时间： | 2000年4月3日 |
IDENTIFICATION OF 4 TRICHINELLA ISOLATES BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS ANALYSIS |
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| SONG Ming-xin,ZHANG Gui-hong,LU Yi-xin,JIANG Cheng-yu.IDENTIFICATION OF 4 TRICHINELLA ISOLATES BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS ANALYSIS[J].Chinese Journal of Veterinary Parasitology,2001,9(1):5-8. |
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| Authors: | SONG Ming-xin ZHANG Gui-hong LU Yi-xin JIANG Cheng-yu |
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| Abstract: | Trichinella from swine and dog in Hei Longjiang province was identified on the basic of Restriction Fragment Length Polymorphisms (RFLP) analysis, the international standard Trichinella isolates: T.spiralis and T.nativa were used as the control. RFLP analysis of 4 Trichinella isolates indicated that swine isolate and T.spiralis were identical, dog isolate and T.nativa were identical, but patterns of the two groups were different to each other. It is concluded that Trichinella from swine and dog belong to T.spiralis and T.nativa respectively. |
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| Keywords: | Trichinella Restriction Fragment Length Polymorphisms |
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