| 5株植物根际促生菌功能特性及培养条件 |
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| 引用本文： | 杨晓蕾,李建宏,姚拓,梅丽娜,李琦,白洁,朱青青,赵晓倩,赵树栋.5株植物根际促生菌功能特性及培养条件[J].草业科学,2022,39(1):30-38. |
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| 作者姓名： | 杨晓蕾 李建宏 姚拓 梅丽娜 李琦 白洁 朱青青 赵晓倩 赵树栋 |
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| 作者单位： | 甘肃农业大学草业学院 / 草业生态系统教育部重点实验室, 甘肃 兰州 730070 |
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| 基金项目： | 国家重点研发计划(2019YFC0507703)%甘肃农业大学人才专项经费(2017RCZX-04) |
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| 摘 要： | 功能特性和培养条件研究是植物根际促生菌(PGPR)工业化潜力挖掘和开发的关键。为了进一步对菌株开发利用,选取前期研究中从珠芽蓼(Polygonum viviparum)、洽草(Koeleria glauca)、红三叶(Trifolium pratense)及草地早熟禾(Poa pratensis)根际获得的5株菌株为材料,测定菌株溶磷、固氮、分泌吲哚乙酸(IAA)能力等特性,利用16S rRNA分子生物学技术,确定菌株分类学地位;测定菌株生长特性、温度耐受性、pH耐受性、产气特性、产酸特性,确定菌株适宜的培养条件。结果表明:菌株M1溶有机磷量最高,为170.37μg·mL?1,菌株Y3溶无机磷量最高,为308.50μg·mL?1;菌株Y1、Y3、Y5、M1、M6均具有分泌IAA的能力,分泌量在24.23~164.74μg·mL?1。菌株Y1和Y3具有固氮能力,固氮酶活性分别为175.30和255.55 nmol·(h·mL)?1(C2H4)。通过对菌株生长特性测定,5株菌株在40 h均已进入生长稳定期,适宜的生长温度在25~31℃,适宜的生长pH范围为6.50~7.50,在LB培养基中生长无产气现象,生长过程中pH稳定在6.23~7.52,具有作为工业化发酵菌种的潜力。16S rDNA鉴定确定菌株Y5与Y3为枯草芽孢杆菌(Bacillus subtilis),Y1为蕈状芽孢杆菌(Bacillus mycoides),M1为产黄假单胞菌(Pseudomonas synxantha),M6为猴假单胞菌属(Pseudomonas simiae)。本研究为菌株的工业化生产研究奠定了基础。
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| 关 键 词： | 植物根际促生菌 固氮 溶磷 培养条件 产气 产酸 菌株鉴定 |
Functional characteristics and culture conditions of five plant growth-promoting rhizobacteria strains |
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| YANG Xiaolei,LI Jianhong,YAO Tuo,MEI Li'na,LI Qi,BAI Jie,ZHU Qingqing,ZHAO Xiaoqian,ZHAO Shudong.Functional characteristics and culture conditions of five plant growth-promoting rhizobacteria strains[J].Pratacultural Science,2022,39(1):30-38. |
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| Authors: | YANG Xiaolei LI Jianhong YAO Tuo MEI Li'na LI Qi BAI Jie ZHU Qingqing ZHAO Xiaoqian ZHAO Shudong |
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| Institution: | (College of Grassland Science,Gansu Agricultural University/Key Laboratory for Grassland Ecosystem of Education Ministry,Lanzhou 730070,Gansu,China;Popularization of Animal Husbandry and Veterinary Technology in Liangzhou District,Wuwei 733000,Gansu,China) |
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| Abstract: | Studying the functional characteristics and culture conditions is essential for successfully exploiting and industrializing plant growth-promoting rhizobacteria(PGPR).To further develop and utilize the strains,five strains obtained from the rhizosphere of Polygonum viviparum,Koeleria glauca,Trifolium pratense,and Poa pratensis were selected as materials in a previous study.The functional characteristics of the strains included the ability to dissolve phosphorus,fix nitrogen,and secrete indoleacetic acid(IAA).The taxonomic status of the strains was confirmed by 16S rDNA molecular biotechnology.The growth characteristics,temperature tolerance,pH tolerance,gas producing characteristics,and acid producing characteristics of the strains were determined,as well as their most appropriate culture conditions.The results showed that M1 had the highest amount of soluble organic phosphorus,170.37μg·mL?1,and Y3 had the highest amount of soluble inorganic phosphorus,308.50μg·mL?1.The strains had the ability to secrete IAA,and the amount secreted was between 24.23 and 104.74μg·mL?1.Furthermore,the Y1 and Y3 strains had the ability to fix nitrogen,and produced 175.30 and 255.55 nmol·(h·mL)?1(C2H4)from nitrogenase activities,respectively.From the determination of the growth characteristics of the strains,it was found that the five strains had entered the stable growth phase at 40 h,the suitable growth temperature was between 25 and 31℃,the suitable growth pH range was between 6.5 and 7.5,and there was no gas production when they were grown in LB medium.The pH was stable between 6.23 and 7.52 during the growth process,indicating their potential as industrial fermentation strains.The 16S rDNA identification confirmed that Y5 and Y3 were Bacillus subtilis,Y1 was Bacillus mycoides,M1 was Pseudomonas synxantha,and M6 was Pseudomonas simiae.This study lays the foundation for the industrial production of the strains. |
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| Keywords: | PGPR nitrogen fixation phosphorus dissolution culture conditions gas production acid production bacterium identification |
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