Quantification of endogenous gibberellins in two flax (Linum usitatissimum L.) cultivars during seed development |
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| Authors: | Sang-Kuk Kim Eun-Young Sohn Sang-Mo Kang In-Jung Lee |
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| Institution: | (1) Plant Molecular Biology Group, Division of Biochemical Sciences, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, 411 008, India;(2) Interactive Research School for Health Affairs, Bharati Vidyapeeth University, Pune, 411043, India;(3) AICRP on Linseed, College of Agriculture, Nagpur, 440 001, India; |
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| Abstract: | Eight gibberellins (GAs) were identified and quantified in extracts of seed of the flax, Linum usitatissimum L. cv. Hwanamama, Hwabaekama by GC-MS-SIM and Kovats retention indices. Four of these gibberellins are members of the early-C13-hydroxylation
pathway (GA53, GA19, GA20, and GA1), and four are members of the non-C13-hydroxylation pathway (GA12, GA24, GA9, and GA4). Of these eight gibberellins, GA24 was more abundant in yellow-seeded flax (cv. Hwanamama) than in brown-seeded flax (cv. Hwabaekama), whereas GA9 was 8.3-fold more abundant in brown-seeded flax (cv. Hwabaekama) than in yellow-seeded flax (cv. Hwanamama). In both flax
cultivars, non-C13-hydroxylation pathway was the main route leading to endogenous accumulation of GA4 in the seed. |
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