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苦瓜活性组分物质抑菌活性测定
引用本文:王贻莲,陈燕平,黄伟,杨合同,李纪顺,司升云,汪钟信.苦瓜活性组分物质抑菌活性测定[J].植物保护,2008,34(2):67-71.
作者姓名:王贻莲  陈燕平  黄伟  杨合同  李纪顺  司升云  汪钟信
作者单位:1. 山东省科学院中日友好生物技术研究中心,山东省应用微生物重点实验室,济南,250014
2. 山东省烟台检验检疫实验中心,264000
3. 山东省济南钢铁股份有限公司技术中心,济南,250101
4. 湖北武汉市蔬菜科学研究所,武汉,430065
5. 华中农业大学,武汉,430070
摘    要:以苦瓜(Momordica charantia L.)种仁中提取的凝集素备提物和苦瓜籽粗蛋白对8种植物病原真菌,5种植物病原细菌进行了抑菌活性测定。结果表明:两种提取物对小麦赤霉病菌、棉花黄萎病菌和苹果炭疽病菌表现出较好的抑菌活性。凝集素备提物(0.67 mg/mL)对小麦赤霉病菌和棉花黄萎病菌的抑菌率分别为69.12%和64.86%;苦瓜籽粗蛋白(6.67 mg/mL)对苹果炭疽病菌的抑菌率达68.61%。两种提取物对大白菜软腐病菌、花生青枯病菌、水稻白叶枯病菌和根癌农杆病菌等植物病原细菌均无抑菌活性,苦瓜籽粗蛋白(0.1 g/mL)对水稻细条病菌有一定抑制作用。

关 键 词:苦瓜凝集素备提物  苦瓜籽粗蛋白  抑菌活性
修稿时间:2007年8月1日

Tests on inhibitory activity of the active components in Momordica charantia L.
Wang Yilian;Chen Yanping;Huang Wei;Yang Hetong;Li Jishun;Si Shengyun;Wang Zhongxin.Tests on inhibitory activity of the active components in Momordica charantia L.[J].Plant Protection,2008,34(2):67-71.
Authors:Wang Yilian;Chen Yanping;Huang Wei;Yang Hetong;Li Jishun;Si Shengyun;Wang Zhongxin
Institution:1.Biotechnology Research Centre of Shandong Academy of Sciences;Key Laboratory of Applied Microbiology of Shandong Province;Jinan 250014;China;2.Yantai Entry-Exit Inspection and Quarantine Bureau;Shandong 264000;China;3.Technology Research Centre of Jinan
Abstract:In order to promote the value of balsam pear,the inhibitory activity of the extracts of MCL and crude proteins to the phytopathogen that was originated from the seeds of balsam pear was tested.The results showed that the extract of MCL had significant inhibitory activity to Gibberella zeae and Verticillium albo-atrum.The highest rate of inhibition was 69.12% and 64.86%,respectively.Protein extracts had significant inhibitory activity to C.gloeosporioides.The highest rate of inhibition was 68.61%.The extracts of MCL and proteins had no inhibitory activities to the pathogenic bacteria in this study,except Xanthomonas campestris pv.oryzicola.
Keywords:extraction of MCL  extraction of crude protein  inhibitory activity
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