Comparative efficacy of standard AGID and precipitinogen inhibition test with monoclonal antibodies based competitive ELISA for the serology of <Emphasis Type="Italic">Peste des Petits Ruminants</Emphasis> in sheep and goats |
| |
| Authors: | Muhammad Munir Muhammad Siddique Qurban Ali |
| |
| Institution: | Department of Veterinary Microbiology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, 38040, Pakistan. muhammad.munir@bvf.slu.se |
| |
| Abstract: | This project was conducted to investigate the comparative efficiency of competitive ELISA (cELISA), standard Agar Gel Immunodiffusion
Test (AGID) and Precipitinogen Inhibition Test (PIT) for the diagnosis of Peste des Petits Ruminants (PPR) in Pakistan. To deal with this, serum samples from 198 sheep and 82 goats were collected from three different government
livestock farms and all the samples were run simultaneously with the three serological tests. The samples found positive for
PPR antibodies through cELISA, AGID and PIT were 96 (34.2%), 60 (21.4%) and 72 (25.7%), respectively. Kappa statistics were
applied to evaluate the concordance between the laboratory-based test (cELISA) and field-based tests (AGID and PIT). Kappa
statistics scores for cELISA versus AGID and PIT were 0.6343 (95% Confidence Interval CI 0.5231–0.7456) and 0.7134 (95% Confidence
Interval CI 0.5987–0.8281), respectively, which indicate a “substantial” agreement between cELISA and AGID and “significant”
agreement between cELISA and PIT. AGID and PIT revealed relative diagnostic sensitivities with cELISA of 59.3% and 69.7% and
relative diagnostic specificities of 98.3% and 97.2%, respectively. The data suggested that for mass screening and control
of PPR, these serological tests proved practical in the absence of cELISA since they have high relative diagnostic specificities
and a satisfactory relative diagnostic sensitivities. |
| |
| Keywords: | Comparison cELISA Peste des Petits Ruminants Serological tests Sheep and goats |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
