| 新型DNA碱基编辑器的研究进展 |
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| 引用本文： | 张雅玲,王锌和,李构思,曾栋昌,祝钦泷,陈乐天,刘耀光.新型DNA碱基编辑器的研究进展[J].华南农业大学学报,2022,43(6):1-16. |
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| 作者姓名： | 张雅玲 王锌和 李构思 曾栋昌 祝钦泷 陈乐天 刘耀光 |
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| 作者单位： | 亚热带农业生物资源保护与利用国家重点实验室/岭南现代农业科学与技术广东省实验室/华南农业大学 生命科学学院, 广东 广州 510642 |
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| 基金项目： | 中国博士后科学基金(2020M682722)；广东省基础与应用基础研究区域联合基金青年项目(2020A1515111101) |
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| 摘 要： | DNA碱基编辑技术是由CRISPR/Cas系统发展而来，能对基因组碱基进行精准编辑。目前已开发的DNA碱基编辑器包括介导C·G至T·A转换的胞嘧啶单碱基编辑器、介导A·T至G·C转换的腺嘌呤单碱基编辑器、介导C·G至G·C颠换的糖基化酶单碱基编辑器、介导C·G至T·A和A·T至G·C同时转换的双碱基编辑器、介导任意碱基之间转换的引导编辑器以及线粒体DNA编辑器。本文系统总结了上述6种DNA编辑器的原理、优化历程及最新研究进展，着重介绍了应用到植物研究中的碱基编辑器工具及其在作物遗传改良中的应用，并对碱基编辑技术今后的发展进行了展望。
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| 关 键 词： | CRISPR/Cas9 碱基编辑技术 DNA碱基编辑器 线粒体DNA 作物改良 |
| 收稿时间： | 2022/8/12 0:00:00 |
Research advances in novel DNA base editors |
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| ZHANG Yaling,WANG Xinhe,LI Gousi,ZENG Dongchang,ZHU Qinlong,CHEN Letian,LIU Yaoguang.Research advances in novel DNA base editors[J].Journal of South China Agricultural University,2022,43(6):1-16. |
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| Authors: | ZHANG Yaling WANG Xinhe LI Gousi ZENG Dongchang ZHU Qinlong CHEN Letian LIU Yaoguang |
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| Institution: | State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Guangdong Laboratory for Lingnan Modern Agriculture/College of Life Sciences, South China Agricultural University, Guangzhou 510642, China |
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| Abstract: | The base editing technology is developed from the CRISPR/Cas gene editing systems, which can perform accurate base or gene editing at the DNA level. Recent years, six types of novel DNA base editors have been developed for the editing of nuclear and organellar genomes, including the cytosine base editor (CBE), the adenine base editor (ABE), the glycosylase base editor (GBE), the adenine and cytosine dual base editor (DBE), the prime editor (PE) and the mitochondrial genome editor. In this review, we summarize the principles, optimization processes and current advances of the above six DNA editors and focus on their application in crop genetic improvement. Finally, the future development of base editing technology is prospected. |
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| Keywords: | CRISPR/Cas9 Base editing technology DNA base editor Mitochondrial DNA Crop improvement |
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