| 膜过滤绿茶多糖的系统分级纯化及免疫活性分析 |
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| 引用本文： | 寇小红,江和源,崔宏春,张建勇,高晴晴,袁新跃,舒爱民,刘晓辉,高琪.膜过滤绿茶多糖的系统分级纯化及免疫活性分析[J].茶叶科学,2008,28(3):172-180. |
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| 作者姓名： | 寇小红 江和源 崔宏春 张建勇 高晴晴 袁新跃 舒爱民 刘晓辉 高琪 |
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| 作者单位： | 1. 农业部茶叶化学工程重点开放实验室,中国农业科学院茶叶研究所,浙江,杭州,310008;中国农业科学院研究生院,北京,100081
2. 农业部茶叶化学工程重点开放实验室,中国农业科学院茶叶研究所,浙江,杭州,310008 |
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| 基金项目： | 国家自然科学基金
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浙江省钱江人才计划 |
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| 摘 要： | 采用150kD、20kD、6kD的超滤膜和对水溶性茶多糖进行分级和浓缩,得到的三部分截留液分别经过DEAE-52纤维素离子交换层析和丙烯葡聚糖凝胶Sephacryl S-300柱层析系统分离、纯化,共得到了20多个分级组分;HPGPC-ELSD鉴定各多糖组分的纯度及分子量分布,结果表明得到5个均一多糖组分;用小鼠巨噬细胞系Raw264.7检测免疫活性,发现对小鼠巨噬细胞Raw264.7有显著活性的多糖组分大多集中在20kD左右,大多为不均一多糖。
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| 关 键 词： | 茶多糖 超滤 分级 纯化 免疫活性 |
| 文章编号： | 1000-369X(2008)03-172-09 |
| 修稿时间： | 2008年3月10日 |
Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities |
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| KOU Xiao-hong,JIANG He-yuan,CUI Hong-chun,ZHANG Jian-yong,GAO Qing-qing,YUAN Xin-yue,SHU Ai-min,LIU Xiao-hui,GAO Qi.Systematic Separation, Purification of Water-soluble Green Tea Polysaccharides and Their Immunological Activities[J].Journal of Tea Science,2008,28(3):172-180. |
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| Authors: | KOU Xiao-hong JIANG He-yuan CUI Hong-chun ZHANG Jian-yong GAO Qing-qing YUAN Xin-yue SHU Ai-min LIU Xiao-hui GAO Qi |
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| Abstract: | Water-soluble green tea polysaccharides were separated and purified by Ultrafiltration membranes with pore diameter of 150 kD, 20 kD, 6 kD in turn. All the three fractions were further separated and purified by DEAE-cellulose ion-exchange chromatography and Sephacryl S-300 column chromatography, and more than 20 green tea polysaccharide components totally were obtained. Their purity and molecular weight distribution were determined with high performance gel permeation chromatography (HPGPC) equipped with ELSD detector, and five components of homogeneous polysaccharide were obtained. The immunological activities of these green tea polysaccharides were determined by stimulated phagocytosis and nitric oxide production on RAW264.7 macrophage cell line of mice. Results showed that the tea Polysaccharides with 20 kD MW possessed significant immunological activities on NO production or phagocytosis of mice macrophage Raw 264.7 cells. |
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| Keywords: | tea polysaccharide ultrafiltration seperation purification immunologic activity |
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