| 丰花月季愈伤组织的诱导及细胞悬浮培养 |
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| 引用本文： | 王艳红,龚束芳,车代弟.丰花月季愈伤组织的诱导及细胞悬浮培养[J].东北农业大学学报,2007,38(2):161-165. |
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| 作者姓名： | 王艳红 龚束芳 车代弟 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 基金项目： | 黑龙江省哈尔滨市科技攻关项目 |
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| 摘 要： | 分别以丰花月季叶片、叶柄和茎段为外植体诱导出愈伤组织,并建立细胞悬浮系。结果表明,丰花月季叶片和茎段是诱导愈伤组织和进行细胞悬浮培养的理想外植体;诱导松散型愈伤组织的最佳激素浓度配比是NAA 4 mg·L-1+2,4-D 1 mg.L-1+BA 1 mg.L-1;细胞悬浮培养细胞最佳起始密度为1.5×104～3.0×104个.mL-1时;继代培养4～5代时,可得到稳定的悬浮细胞系。
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| 关 键 词： | 丰花月季 组织培养 细胞悬浮培养 松散型愈伤组织 |
| 文章编号： | 1005-9369（2007）02-0161-05 |
| 收稿时间： | 2005-09-21 |
| 修稿时间： | 2005-09-21 |
Study on callus induction and cell suspension cultures of rose (Rosa floribunda) |
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| WANG Yanhong,GONG Shufang,CHE Daidi.Study on callus induction and cell suspension cultures of rose (Rosa floribunda)[J].Journal of Northeast Agricultural University,2007,38(2):161-165. |
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| Authors: | WANG Yanhong GONG Shufang CHE Daidi |
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| Institution: | College of Horticulture, Northeast Agricultural University, Harbin 150030, China |
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| Abstract: | Cell suspension cultures were established from the callus tissues which were induced from leaf,leafstalk and stem segments of Rosa floribunda.The result indicated that both leaf and stem explants were suitable for cell suspension.Friable callus was obtained when cultured on Murashige and Skoog(MS) medium supplemented with 4 mg·L-1 NAA,1 mg·L-1 2,4-dichlorophenoxyacetic acid(2,4-D),1 mg·L-1 6-benzyl-adenine(6-BA).The best origination density was 1.5×104-3.0×104 number·mL-1;after 4-5 generations of sub-culture,stable cell suspension was obtained. |
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| Keywords: | Rosafloribunda tissue culture cell suspension cultures friable callus |
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