| 利用16S rRNA快速鉴定双歧杆菌的研究 |
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| 引用本文： | 徐文生,陈湘宁,艾启俊,刘一清,庞美霞.利用16S rRNA快速鉴定双歧杆菌的研究[J].北京农学院学报,2006,21(2):44-46. |
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| 作者姓名： | 徐文生 陈湘宁 艾启俊 刘一清 庞美霞 |
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| 作者单位： | 北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206;北京农学院,食品科学系,北京,102206 |
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| 基金项目： | 北京市科委资助项目(2004B14) |
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| 摘 要： | 为了建立从生物制品和人粪便中快速分离鉴定双歧杆菌的方法,利用双歧杆菌16S rRNA特异性扩增引物,通过PCR技术对双歧杆菌制剂和人粪便中分离菌种进行快速鉴定;同时结合革兰氏染色、穿刺实验、糖发酵实验等技术对分离的双歧杆菌的生理生化等特征进行了研究。琼脂糖电泳结果表明,利用特异性扩增引物的扩增产物在1 500 bp处出现特异性条带,革兰氏染色、糖发酵试验等也验证了分离菌种为双歧杆菌。说明利用16S rRNA的PCR方法鉴定双歧杆菌具有快速、灵敏、操作简单等优点,可以直接将双歧杆菌快速鉴定到属。
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| 关 键 词： | 双歧杆菌 鉴定 多聚酶链式反应 |
| 文章编号： | 1002-3186(2006)02-0044-03 |
| 收稿时间： | 2005-12-22 |
| 修稿时间： | 2006-03-22 |
Rapid Identification of Bifidobacterium by Using 16S rRNA |
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| XU Wen-sheng,CHEN Xiang-ning,AI Qi-jun,LIU Yi-qing,PANG Mei-xia.Rapid Identification of Bifidobacterium by Using 16S rRNA[J].Journal of Beijing Agricultural College,2006,21(2):44-46. |
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| Authors: | XU Wen-sheng CHEN Xiang-ning AI Qi-jun LIU Yi-qing PANG Mei-xia |
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| Institution: | Department of Food Science and Technology,Beijing Agricultural College, Beijing 102206, China |
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| Abstract: | The rapid method was developed to identify Bifidobacterium from biologicals preparations and human feces.Using the special primers to amplify the 16S rDNA by PCR,the Bifidobacterium in Biological preparations or human feces was rapidly identified.At the same time,the physiological and biochemical characteristics of Bifidobacterium were studyed by methods such as Gram's staining,carbohydrate fermentation test,puncture test,determination of growth curve,etc.Results demonstrated that the isolated strains were Bifidobacterium.This paper showed that identification of Bifidobacteria could be simply fast completed with the PCR amplification of the 16S rDNA. |
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| Keywords: | Bifidobacterium identification PCR |
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