| 棕榈科植物SSR分子标记反应体系的建立 |
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| 引用本文： | 符海泉,杨耀东,符海瑜,万婕,马子龙,吴翼.棕榈科植物SSR分子标记反应体系的建立[J].江西农业学报,2013(11):6-10. |
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| 作者姓名： | 符海泉 杨耀东 符海瑜 万婕 马子龙 吴翼 |
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| 作者单位： | [1]海南省热带油料作物生物学重点实验室／中国热带农业科学院椰子研究所,海南文昌571339 [2]江西中医学院科技学院,江西南昌330025 [3]中国热带农业科学院热带生物技术研究所,海南海口571101 |
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| 基金项目： | 海南省自然科学基金（No.312039）. |
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| 摘 要： | 以棕榈科植物的DNA为材料，通过对SSR反应体系中模板DNA、dNTPs、引物、Taq聚合酶和MgCl25个因素采用单因素试验法设定5个梯度，并比较不同的浓度、不同的用量对扩增效果的影响，建立最佳反应体系。研究最终确定了20μLPCR反应体系的最佳条件为：模板DNA2μL，dNTPs0．2μL，引物1．0μL，Taq聚合酶O．2μL，MgCl22．0μL。该优化体系稳定可靠，适合应用于棕榈科植物SSR分析。
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| 关 键 词： | 棕榈科 SSR 优化 |
Establishment of SSR -PCR Reaction System for Palm Plants |
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| FU Hai-quan,YANG Yao- dong,FU Hai-yu,WAN Jie MA Zi-long,WU Yi.Establishment of SSR -PCR Reaction System for Palm Plants[J].Acta Agriculturae Jiangxi,2013(11):6-10. |
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| Authors: | FU Hai-quan YANG Yao- dong FU Hai-yu WAN Jie MA Zi-long WU Yi |
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| Institution: | 1. Hainan Provincial Key Laboratory of Tropical Oil Crops Biology/Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wenchang 571339, China; 2. College of Science and Technology, Jiangxi University of Traditional Chinese Medicine, Nanchang 330025, China; 3. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricul- tural Sciences, Haikou 571101, China) |
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| Abstract: | To establish the optimal SSR - PCR reaction system for palm trees, different factors that mostly affected the amplifi- cation of SSR - PCR reaction, including DNA template, dNTPs, primers, TaqDNA and MgC12, were investigated and optimized by setting up 5 gradients with single - fact test method in the experiment. Meanwhile, we also compared the influences of different con centrations and different consumptions on the amplification. The results showed that the optimal SSR - PCR system was DNA template 2 μL, dNTPs 0.2 μL, primers 1.0 μL, TaqDNA 0.2 μL, and MgC12 2.0 μL, which was more stable and suitable for the SSR analysis of palm plants. |
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| Keywords: | Palm tree SSR Optimization |
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