| 西藏小麦品种籽粒硬度遗传多样性 |
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| 引用本文： | 李善富,李建民,王雪玲,魏 乐,李红琴,刘宝龙,张怀刚.西藏小麦品种籽粒硬度遗传多样性[J].西北农业学报,2014,23(8):40-44. |
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| 作者姓名： | 李善富 李建民 王雪玲 魏 乐 李红琴 刘宝龙 张怀刚 |
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| 作者单位： | (1.中国科学院 西北高原生物研究所，西宁 810001；2.青海师范大学 生命与地理科学学院，西宁 810001；3.中国科学院 高原生物适应与进化重点实验室,西宁 810001；4.青海省作物分子育种重点实验室,西宁 810001） |
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| 基金项目： | 青海省高原作物种质资源创新与利用国家重点实验室培育基地开放课题（2011-07）；中国科学院知识创新工程重要方向项目(KSCX2-EW-N-02)；国家自然科学基金地区基金（31260322）。 |
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| 摘 要： | 籽粒硬度是影响小麦磨粉品质和食品品质的重要因素。利用单粒谷物特性测定仪、PCR扩增和核苷酸测序技术,对121份西藏地方品种进行籽粒硬度性状遗传多样性研究。结果表明:西藏品种籽粒硬度平均值为43.73%,硬度值大于60%的品种有43种,占35.5%;混合麦22个,占18.2%;软质麦56个,比例为46.3%。符合优质饼干硬度特性的品种有42种,达到34.7%。有5种硬度基因组合类型:野生型、PinaD1b、Pinb-D1b、Pinb-D1c、Pinb-D1p。野生型比例最高,占51.22%。Pinb-D1c次之,占21.31%,其余依次为Pinb-D1b、Pina-D1b、Pinb-D1p。各种组合类型的SKCS硬度值Pinb-D1cPina-D1bPinb-D1bPinb-D1p野生型。西藏小麦品种籽粒硬度性状遗传多样性的研究将为青藏高原以及其他地区的育种提供种质资源和理论依据。
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| 关 键 词： | 小麦 籽粒硬度 Puroindoline 遗传多样性 西藏 |
Genetic Variation of Grain Hardness of Wheat Cultivars in Tibet |
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| LI Shanfu,LI Jianmin,WANG Xueling,WEI Le,LI Hongqin,LIU Baolong and ZHANG Huaigang.Genetic Variation of Grain Hardness of Wheat Cultivars in Tibet[J].Acta Agriculturae Boreali-occidentalis Sinica,2014,23(8):40-44. |
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| Authors: | LI Shanfu LI Jianmin WANG Xueling WEI Le LI Hongqin LIU Baolong and ZHANG Huaigang |
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| Abstract: | Grain hardness was a key factor deciding wheat milling quanlity and end-used food quanlity. In this study, single kernel characterization system(SKCS), PCR and nucleotide sequencing were employed for lighting genetic diversity of grain hardness of Tibet wheat cultivars. Average hardness index of all cultivars was 43.73%. 43 cultivars had hardness index greater than 60%, accounting for 35.5% of total cultivars; 22 cultivars were mixed wheat, accounting for 18.2%, and 56 cultivars were soft wheat, accounting for 46.3%. 42 cultivars had hardness index less than 30, which means they can be good candidates for biscuit. Five genotypes of puroindoline gene combinations existed in these cultivars. They were wild type, Pina-D1b, Pinb-D1b, Pinb-D1c and Pinb-D1p . The proportion of wild type was the highest, and reached 51.22%, Pinb-D1c was the second, and reached 21.31%, while Pinb-D1b, Pina-D1b and Pinb-D1p followed. The order of SKCS value of these genotypes was Pinb-D1c>Pina-D1b>Pinb-D1b>Pinb-D1p>wild type. These results could provide materials and theoretical base for wheat breeding in Tibet and other provinces. |
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| Keywords: | Wheat Grain hardness Puroindoline Genetic variation Tibet |
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