| 月季切花衰老相关基因的差异显示及其序列分析 |
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| 引用本文： | 白双义,刘青林,欧阳青,蔡文启.月季切花衰老相关基因的差异显示及其序列分析[J].农业生物技术学报,2003,11(2):154-157. |
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| 作者姓名： | 白双义 刘青林 欧阳青 蔡文启 |
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| 作者单位： | 1. 中国农业大学观赏园艺与园林系,北京,100094
2. 中国科学院微生物研究所,北京,100080 |
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| 摘 要： | 摘要：通过对不同瓶插寿命切花月季（Rosa hybrida）品种的DDRT-PCR方法，从长寿命的Pavarotti花瓣中扩增出22条cDNA特异片段，共得到9个cDNA片段的克隆，分别命名为b21、b32（b33）、b43、b51（b52、b814）、b618（b101、b108、b813）、b624、b131、b134和b186。序列测定和同源性检索的结果表明，来自b6的2个克隆b618和b624的序列不同，其中一个片段b618为439 bp，其氨基酸序列与拟南芥（Arabisopsis thaliana）、柑橘（Citrus sinensis）、萝卜（Raphanus sativus）、苹果（Malus sp.）、葡萄（Vitis vinifera）和胡颓子（Elaeagnus umbellata）等查尔硐异构酶(CHI)相应区域的同源性为71%~79%；另一个片段b624为440 bp,其氨基酸序列与拟南芥、豌豆（Pisum sativum）、欧洲油菜（Brassica napus）等的磷酸乙酰胆碱胞苷转移酶(CPCT)相应区域的同源性高达86%~90%。RT-PCR验证表明这2条cDNA片段为阳性结果。CHI涉及类黄酮色素的生物合成途径，CPCT参与胞苷二磷酸胆碱的合成途径，二者均与衰老进程相关。
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| 关 键 词： | 关键词：月季切花 DDRT-PCR 衰老相关基因 查尔酮异构酶 磷酸乙酰胆碱胞苷酰转移酶 |
| 修稿时间： | 2002年1月14日 |
Differential Display and Sequence Analysis of Senescence-associated Genes in Cut Roses |
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| Abstract: | Abstract: Using DDRT-PCR, twenty-two cDNA specific fragments were amplified from petal total RNA of Rosa cv. Pavarotti with longer vase life. Nine of those cDNA specific fragments were cloned, which were b21 , b32（b33）, b43 , b51（b52 , b814）, b618（b101 , b108 , b813）, b624 , b131 , b134 and b186 respectively. Sequences blasted in GenBank showed that b618 and b624 derived from recombination plasmid b6 were two different sequences. b618 , which had 439 bp and 137 deduced amino acid, was 71%~79% homologous to that of corresponding encoding regions of chalcone isomerase(CHI), such as Arabisopsis thaliana, Citrus sinensis, Raphanus sativus, Malus sp., Vitis vinifera and Elaeagnus umbellata . While b624 , which had 440 bp and 134 deduced amino acid,was 86%~90% identical to the cholinephosphate cytidylyltransferase (CPCT) with Arabidopsis thaliana, Pisum sativum and Brassica napus. RT-PCR showed b618 and b624 were positive results. CHI was an enzyme involved in flavonoid biosynthesis pathway, while CPCT is responsible to CDP-choline pathway, both were related to the senescence process. |
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| Keywords: | DDRT-PCR |
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