| 大白菜SRAP-PCR反应体系的优化 |
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| 引用本文： | 徐莹莹,屈淑平,崔崇士.大白菜SRAP-PCR反应体系的优化[J].东北农业大学学报,2008,39(8). |
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| 作者姓名： | 徐莹莹 屈淑平 崔崇士 |
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| 作者单位： | 东北农业大学园艺学院,哈尔滨,150030 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 对大白菜DNA的SRAP-PCR扩增体系进行优化,为大白菜抗软腐病基因图谱的构建和分子标记奠定基础。以大白菜基因组DNA为模板,对PCR反应体系的各影响因子进行梯度试验,筛选可扩增多态性高、重复性好、带型清晰的最佳体系。该体系(25μL)为:Mg2+3.0 mmol·L-1、dNTPs 0.3 mmol·L-1、Taq酶1.5 U、引物0.2μmol·L-1、模板DNA 60 ng。该体系能很好地满足大白菜基因组SRAP扩增的要求,SRAP标记应用于大白菜遗传研究是可行的。
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| 关 键 词： | 大白菜 SRAP 体系优化 |
Optimization of SRAP-PCR program and system in Chinese cabbage |
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| XU Yingying,QU Shuping,CUI Chongshi.Optimization of SRAP-PCR program and system in Chinese cabbage[J].Journal of Northeast Agricultural University,2008,39(8). |
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| Authors: | XU Yingying QU Shuping CUI Chongshi |
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| Institution: | XU Yingying,QU Shuping,CUI Chongshi(College of Horticulture,Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The aim of the research is to establish SRAP-PCR amplification system,which is suitable for Chinese cabbage DNA so as to lay foundation for the construction of gene map and molecular marker in Chinese cabbage.Using Chinese cabbage genome DNA as template,the gradient experiment was conducted on each impact factors in PCR reaction system to screen and establish the optimum system that could amplify high polymorphism,good repeat ability and clear band pattern.The optimum system(total of 25 μL) was as follows: Mg2+ 3.0 mmol·L-1,dNTPs 0.3 mmol·L-1,Taq polymerase 1.5 U,primer 0.2 μmol·L-1,DNA 60 ng.The system could meet the demands for genome SRAP amplification in Chinese cabbage.It is feasible to apply SRAP marker in genetic research in Chinese cabbage. |
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| Keywords: | SRAP |
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