Fusarium vascular wilt in lentil: Inheritance and identification of DNA markers for resistance |
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| Authors: | I Eujayl W Erskine B Bayaa M Baum E Pehu |
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| Institution: | International Centre for Agricultural Research in the Dry Areas, Germplasm Program PO Box 5466, Aleppo, Syria;Faculty of Agriculture, University of Aleppo, Aleppo, Syria;Department of Plant Production, PL 27 (Viikki, Ctalo) FIN 00014, University of Helsinki, Finland |
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| Abstract: | The inheritance of resistance to lentil (Lens culinaris Medik.) vascular wilt caused by Fusarium oxysporum f.sp. lentis was investigated in a cross between resistant (ILL5588) and susceptible (L692–16-l(s)) lines. F2:4 progenies and F6:8, F6:9 recombinant inbred line (RIL) populations were assessed for their wilt reaction for three seasons in a well-established wilt-sick plot. Resistance to wilt was conditioned by a single dominant gene in the populations studied. The map location of the Fw locus was identified for the first time through linkage to a random amplified polymorphic DNA (RAPD) marker (OPK-15900) at 10.8 cM. Two other RAPD markers (OP-BH800 and OP-DI5500) identified by bulked segregant analysis were associated in the coupling phase with the resistance trait, and another marker (OP-C04650) was associated with repulsion. The DNA markers reported here will provide a starting point in marker-assisted selection for vascular wilt resistance in lentil. |
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| Keywords: | Lens culinaris Fusarium oxysporum f sp lentis RAPD markers recombinant inbred lines resistance |
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