| 病原菌在红壤胶体上的吸附机制研究 |
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| 引用本文： | 赵文强,刘星,蔡鹏,黄巧云.病原菌在红壤胶体上的吸附机制研究[J].土壤学报,2013,50(2):221-229. |
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| 作者姓名： | 赵文强 刘星 蔡鹏 黄巧云 |
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| 作者单位： | 1. 华中农业大学农业微生物学国家重点实验室,武汉,430070
2. 华中农业大学农业微生物学国家重点实验室,武汉430070;华中农业大学资源与环境学院,农业部长江中下游耕地保育重点实验室,武汉430070 |
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| 基金项目： | 国家自然科学基金项目(41171196、40825002)、国家高技术研究发展计划（2009AA06Z302)和全国优秀博士学位论文作者专项资金(201066)资助 |
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| 摘 要： | 研究了pH和KCl离子强度对猪链球菌和大肠杆菌在红壤胶体表面吸附的影响,结合表面物化性质和Derjaguin-Landau-Verwey-Overbeek(DLVO)理论分析互作机制。结果表明,细菌在红壤胶体表面的吸附等温线能较好拟合Freundlich方程(R20.97),猪链球菌在红壤胶体表面吸附的分配系数(Kf)是大肠杆菌的4.5倍～6.1倍,细菌在去有机质胶体表面吸附的Kf值为含有机质胶体的2.4倍～3.2倍。比表面积越大或zeta电位绝对值越小,细菌吸附能力越强,吸附态细菌位于距红壤胶体表面90～100 nm处的次级小能位置。随着体系pH降低(9.0～4.0)或离子强度增大(1～10 mmol L-1),细菌与红壤胶体互作能障降低,细菌吸附量增大,吸附机制符合DLVO理论;而在高离子强度下(50～100 mmol L-1),猪链球菌吸附量降低了3.4%～5.6%,表明除DLVO作用力外,非DLVO作用力如空间位阻排斥和疏水作用对吸附也有贡献。
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| 关 键 词： | 红壤胶体 猪链球菌 大肠杆菌 吸附 DLVO理论 |
| 收稿时间： | 2012/6/21 0:00:00 |
| 修稿时间： | 2012/9/17 0:00:00 |
Mechanisms of Bacterial Pathogens Adsorption on Red Soil Colloids |
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| Zhao Wenqiang,Liu Xing,Cai Peng and Huang Qiaoyun.Mechanisms of Bacterial Pathogens Adsorption on Red Soil Colloids[J].Acta Pedologica Sinica,2013,50(2):221-229. |
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| Authors: | Zhao Wenqiang Liu Xing Cai Peng and Huang Qiaoyun |
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| Institution: | State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University; Key Laboratory of Arable Land Conservation in Middle and Lower Reaches of Yangtse River, Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University,State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University; Key Laboratory of Arable Land Conservation in Middle and Lower Reaches of Yangtse River, Ministry of Agriculture, College of Resources and Environment, Huazhong Agricultural University |
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| Abstract: | Effects of soil pH and ionic strength (KCl) on adsorption of Streptococcus suis and Escherichia coli on Red soil colloids were studied, and mechanisms of their interactions analyzed from the aspects of surface physicochemical properties of the bacteria and soil colloids and Derjaguin-Landau-Verwey-Overbeek (DLVO) theory. Results show that the adsorption isotherms of bacteria on Red soil colloids fitted well to the Freundlich equation (R2> 0.97). The partition coefficients (Kf) of S. suis adsorption on soil colloids were 4.5~6.1 times as high as those of E. coli. The Kf values of organic-matter-depleted colloids were 2.4~3.2 times as high as those of organic-matter-containing colloids. The bigger the specific surface areas or the lower the absolute zeta potential values, the higher the bacteria adsorption capacity, and the adsorbed bacteria were found at the secondary minimum, 90~100 nm apart from the colloids surface. With decreasing solution pH (9.0~4.0) or increasing IS (1~10 mmol L-1), the interaction energy barrier between the Red soil colloids and the bacteria decreased and as a result more and more bacteria got adsorbed. This trend was in full agreement with the DLVO theory. The adsorption of S. suis on colloids decreased by 3.4%~5.6% under high IS (50~100 mmol L-1), which, besides the DLVO effect, was also ascribed to some non-DLVO effects, like steric repulsion and hydrophobic interaction. |
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| Keywords: | Red soil colloids Streptococcus suis Escherichia coli Adsorption DLVO theory |
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