| 马铃薯幼苗在冷驯化期间的生理生化变化 |
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| 引用本文： | 李飞,刘杰,段绍光,金黎平.马铃薯幼苗在冷驯化期间的生理生化变化[J].中国马铃薯,2008,22(5). |
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| 作者姓名： | 李飞 刘杰 段绍光 金黎平 |
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| 作者单位： | 1. 中国农业科学院蔬菜花卉研究所,北京,100081;贵州省马铃薯研究所,贵州,贵阳,550006
2. 中国农业科学院蔬菜花卉研究所,北京,100081 |
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| 基金项目： | 国家科技支撑计划农业领域课题 |
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| 摘 要： | 以霜冻敏感的中薯3号和耐冻的野生马铃薯03079-435(Solanum acaule)的幼苗为试材,对其总叶绿素含量、丙二醛(MDA)含量、脯氨酸含量、可溶性糖含量、可溶性蛋白含量的变化进行测定。结果表明:冷驯化期间(4℃/2℃,昼/夜),两者的总叶绿素含量都呈先降后升的变化趋势;03079-435叶片的脯氨酸含量先升高后降低,总体呈升高趋势,而中薯3号叶片的脯氨酸含量逐渐降低;两个基因型的可溶性糖和可溶性蛋白含量均有所升高,中薯3号叶片的可溶性蛋白含量增加幅度明显低于03079-435。推断幼苗叶片脯氨酸和可溶性蛋白含量的增加与冷驯化能力的增强密切相关。
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| 关 键 词： | 马铃薯幼苗 冷驯化 生理生化变化 |
Physic-biochemical Changes Related to the Freezing Tolerance during Cold Acclimation in Potato Seedlings |
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| Li Fei,Liu Jie,Duan Shaoguang,Jin Liping.Physic-biochemical Changes Related to the Freezing Tolerance during Cold Acclimation in Potato Seedlings[J].Chinese Potato,2008,22(5). |
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| Authors: | Li Fei Liu Jie Duan Shaoguang Jin Liping |
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| Abstract: | The chlorophyll content,MDA content,proline content,the soluble sugar content,and the soluble protein content were determined in the freezing sensitive variety Zhongshu 3(Solanum tuberosum)and freezing tolerant clone 03079-435(S.acaule)in order to understand the effects of cold acclimation on physio-biochemical changes in potato.The chlorophyll contents of both 03079-435 and Zhongshu 3 decreased during the period of cold acclimation(day/night temperature 4℃/2℃).The proline content of 03079-435 increased first and then decreased but more than that before cold acclimation,while the proline content of Zhongshu 3 was decreasing.The content of soluble sugar of the two genotypes increased,however the content of soluble sugar of 03079-435 was more than that of Zhongshu 3 during cold acclimation.These data suggest that increases in proline and soluble protein content be closely associated to the high cold acclimation in potato. |
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| Keywords: | potato seedling cold acclimation physic-biochemical changes |
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