| 植物乳杆菌Lp基因表达时内参基因的选择 |
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| 引用本文： | 樊磊,于长青,姚笛,张明玉,李琳.植物乳杆菌Lp基因表达时内参基因的选择[J].黑龙江八一农垦大学学报,2016(5). |
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| 作者姓名： | 樊磊 于长青 姚笛 张明玉 李琳 |
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| 作者单位： | 黑龙江八一农垦大学食品学院,大庆,163319 |
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| 基金项目： | 黑龙江省自然基金重点项目资助(ZD201207)；黑龙江省博士后专项经费资助(LBH-Q13133)。 |
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| 摘 要： | 实验以植物乳杆菌Lp为实验材料,用荧光定量PCR技术分析16S rRNA、Glyceraldehyde-3-phosphate dehydrogenas、L-lactate dehydrogenase以及rec A protein四个候选内参基因的表达情况。应用两种软件ge Norm和Norm Finder程序对实验结果进行分析。实验结果显示在植物乳杆菌Lp中,稳定值最小的是16S ribosomal RNA,用Norm Finder软件分析,稳定值最小的也是16S ribosomal RNA。揭示16S ribosomal RNA适合做植物乳杆菌正常生长状态下的内参基因。
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| 关 键 词： | 实时荧光定量PCR 植物乳杆菌Lp 内参基因 |
Reference Gene Selection of Gene Expression in Lactobacillus Plantarum Lp |
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| Abstract: | The fluorescence quantitative PCR was used to detect the gene expression level of four genes(16S rRNA,Glyceraldehyde-3-phosphate dehydrogenas,L-lactate dehydrogenase and recA protein)in Lactobacillus plantarum Lp. The experimental results were analyzed with two kinds of software geNorm and NormFinder program. The result showed that the stable minimum value was 16s rRNA in the two software. 16s rRNA was suitable for the reference gene in Lactobacillus plantarum Lp. |
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| Keywords: | real-time fluorescence quantitative PCR Lactobacillus plantarum Lp reference gene |
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