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| 农杆菌介导籼稻转化体系的优化 | |
| 引用本文： | 潘素君,戴良英,刘雄伦,张昊,王国梁.农杆菌介导籼稻转化体系的优化[J].核农学报,2006,20(4):263-268. |
| 作者姓名： | 潘素君 戴良英 刘雄伦 张昊 王国梁 |
| 作者单位： | 1. 湖南农业大学生物安全科技学院,湖南,长沙,410128;湖南农业大学水稻基因组学实验室,湖南,长沙,410128 2. 湖南农业大学水稻基因组学实验室,湖南,长沙,410128 3. 湖南农业大学水稻基因组学实验室,湖南,长沙,410128;美国俄亥俄州立大学植物生理实验室,43210 |
| 基金项目： | 国家科技攻关项目;国家自然科学基金;湖南农业大学校科研和教改项目 |
| 摘 要： | 以8个籼稻品种作材料,研究了携带抗稻瘟病基因Pi9片段的pCAMBIA1301质粒的根癌农杆菌EHA105转化籼稻幼胚愈伤组织的影响因素。结果表明,NB培养基适用于籼稻愈伤组织的诱导,共培养时间以3d为宜。40mg/L的潮霉素可用于籼稻愈伤组织的筛选,抗性愈伤率在69.4%~85.3%之间;抗性愈伤组织经分化培养,幼苗分化率在15.4%~47.4%;鉴于多数籼稻愈伤组织分化时对潮霉素特别敏感,因此分化培养基中不加潮霉素;但为了减少假阳性,生根培养基中分别用20mg/L和30mg/L的潮霉素对幼苗进行筛选。 |
| 关 键 词： | 籼稻 抗性愈伤 分化 潮霉素 |
| 文章编号： | 1000-8551(2006)04-263-06 |
| 收稿时间： | 03 28 2006 12:00AM |
| 修稿时间： | 2006年3月28日 |
OPTIMIZATION OF TRANSFORMATION MEDIATED BY Agrobacterium IN Indica RICE |
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| Pan Su-jun,Dai Liang-ying,Liu Xiong-lun,Zhang Hao,Wang Guo-liang.OPTIMIZATION OF TRANSFORMATION MEDIATED BY Agrobacterium IN Indica RICE[J].Acta Agriculturae Nucleatae Sinica,2006,20(4):263-268. | |
| Authors: | Pan Su-jun Dai Liang-ying Liu Xiong-lun Zhang Hao Wang Guo-liang |
| Abstract: | Factors influencing the frequency of rice transformation mediated by A. tumefacience strain EHA105 harboring pCAMBIA1301 plasmid carrying blast-resistant Pi9 gene were investigated on 8 lndica rice cultivars. The result showed that NB medium was suitable for calli induction of lndica rice, the best time for the co-culture of callus and Agrobacterium was 3 days, 40mg/L hygromycin was fit for resistant selection, 69.4% - 85.3% resistant calli survived. In differentiation medium, the frequencies of eight lndica rice varieties were between 15.4% - 47.4%. Most of lndica rice calli were sensitive to hygromycin in differention medium, hygromycin was unfit for differention. In order to reduce false positive plants, we used 20mg/L and 30mg/L hygromycin to select the young green plants. |
| Keywords: | Indica rice resistant calli differentiation hygromycin |
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