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不同测定方法测定小蓟中绿原酸含量的研究
引用本文:周建青,徐爱列,王国锋.不同测定方法测定小蓟中绿原酸含量的研究[J].安徽农业科学,2009,37(27):12902-12903.
作者姓名:周建青  徐爱列  王国锋
作者单位:青海师范大学化学系,青海西宁,810008;青海师范大学化学系,青海西宁,810008;青海师范大学化学系,青海西宁,810008
摘    要:目的]选用分光光度法与HPLC法同时测定青海小蓟中绿原酸的含量,以寻找既迅速快捷又廉价的检测方法。方法]采用甲醇对小蓟中绿原酸进行提取,①采用酚类物质中酚羟基与三氯化铁的显色反应,进行绿原酸含量的比色测定;②用高效液相色谱仪(HPLC)对小蓟中的绿原酸含量进行测定。结果]小蓟中绿原酸含量以HLPC法测出的值较高。结论]这两种测定方法简单、快捷,均可用于小蓟中绿原酸含量的检测与评价。

关 键 词:可见分光光度计  HPLC  小蓟  绿原酸

Study on the Determination Methods of Chlorogenic Acids from Cephalanoplos segetum
ZHOU Jian-qing et al.Study on the Determination Methods of Chlorogenic Acids from Cephalanoplos segetum[J].Journal of Anhui Agricultural Sciences,2009,37(27):12902-12903.
Authors:ZHOU Jian-qing
Institution:ZHOU Jian-qing et al(Department of Chemistry,Qinghai Normal University,Xining,Qinghai 810008)
Abstract:Objective] The chlorogenic acid content in Cephalanoplos segetum in Qinghai was determined by using 721 spectrophotometer and HPLC to seek the rapid and cheap determination methods.The content of effective component chlorogenic acid in Cephalanoplos segetum was determined.Method] Chlorogenic acids were extracted from Cephalanoplos segetum by methanol extraction with two determination methods.Based on the color reaction of phenolic compounds with phenolic hydroxy group and iron trichloride,chlorogenic acid...
Keywords:HPLC
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