| 山东莱州发现三倍体薤白 |
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| 引用本文： | 王爱云,王艳化,任晓莉,张琳丰.山东莱州发现三倍体薤白[J].农业科学与技术,2009,10(1):124-126. |
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| 作者姓名： | 王爱云 王艳化 任晓莉 张琳丰 |
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| 作者单位： | 鲁东大学生命科学学院,山东烟台,264025
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| 基金项目： | 鲁乐大学校基金资且项目 |
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| 摘 要： | 1材料与方法
供试薤白取自山东省莱州市郊外。取薤白的根尖用0．002mol／L 8-羟基喹啉溶液预处理4h，卡诺氏液在冰箱中固定24h，以1mol／L的盐酸60℃解离6～9min，再用改良苯酚品红染色液染色后制片。镜检并统计染色体数目，然后选用染色体形态好且分散的细胞，在配有Olympus DV70数码摄像头的多功能系统显微镜（Olympus BX50）下拍照分析。核型分析按照李懋学、陈瑞阳报道的标准进行，核型分类参照Stebbins GL的方法划分。
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| 关 键 词： | 山东省 薤白 莱州 三倍体 Olympus 8-羟基喹啉 染色体数目 染色体形态 |
Discovery of Triploid Allium macrostemon Bunge in Laizhou City of Shandong Province |
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| WANG Ai-yun,WANG Yan-hua,REN Xiao-li,ZHANG Lin-feng.Discovery of Triploid Allium macrostemon Bunge in Laizhou City of Shandong Province[J].Agricultural Science ＆ Technology,2009,10(1):124-126. |
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| Authors: | WANG Ai-yun WANG Yan-hua REN Xiao-li ZHANG Lin-feng |
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| Institution: | ( College of Life Science, Ludong University, Yantai 264025) |
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| Abstract: | Objective] The study was to analyze the karyotype of Allium macrostemon Bunge in Laizhou City of Shandong Province. Method] The root tip of A.macrostemon was pretreated with 8-hydroxyquinoline solution, fixed, dissociated and stained for preparing the glass slide to reveal the chromosome number via the microscopic examination; the sparse cells with good chromosome morphology were photographed under microscope. Result] Allium macrostemon Bunge in Laizhou City introduced in this study was triploid; its somatic chromosome number was 24 and karyotype formula was K(2n)=3x=24m(2SAT)+1Bs, thus the karyotype belongs to 1A type. One of the chromosome No. 3 contained satellite, and chromosome deletion may be existed in one of the chromosome No. 5. In addition, B chromosome was observed in some cells. Conclusion] This introduction of triploid A.macrostemon found in China was the first time. |
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| Keywords: | Allium macrostemon Bunge Karyotype analysis Triploid |
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