| 屠宰牛卵母细胞体外成熟和体外受精研究 |
| |
| 引用本文： | 严光文,殷红梅,刘长松.屠宰牛卵母细胞体外成熟和体外受精研究[J].中国奶牛,2008(1):28-32. |
| |
| 作者姓名： | 严光文 殷红梅 刘长松 |
| |
| 作者单位： | 1. 塔里木大学动物科技学院,阿拉尔843300;四川农业大学动物科技学院,雅安,625014
2. 四川农业大学动物科技学院.雅安,625014 |
| |
| 摘 要： | 对采集到的卵巢采取抽吸法、割破法回收卵母细胞，割破法得到的A级卵母细胞显著高于抽吸法。把有黄体和无黄体的卵巢卵母细胞进行培养比较，无黄体的卵巢卵母细胞的成熟率为84．33％，显著高于有黄体卵巢卵母细胞的74．32％。本试验中卵泡卵母细胞和深层卵母细胞体外成熟率均能达到75％以上．卵巢表面卵泡卵母细胞的卵裂率为54．76％，高于深层卵母细胞的卵裂率，但二者无显著差异。IVM时添加卵丘细胞、IVC时添加输卵管上皮细胞是比较理想的培养系统。
|
| 关 键 词： | 卵母细胞 体外受精 体外成熟 体外培养 |
| 文章编号： | 1004-4264（2008）01-0028-04 |
| 收稿时间： | 2007-09-06 |
| 修稿时间： | 2007年9月6日 |
Study on in Vitro Maturation and in Vitro Fertilization of Bovine Oocyte from Dead Body |
| |
| Yan Guangwen,Yin Hongmei,Liu Changsong.Study on in Vitro Maturation and in Vitro Fertilization of Bovine Oocyte from Dead Body[J].China Dairy Cattle,2008(1):28-32. |
| |
| Authors: | Yan Guangwen Yin Hongmei Liu Changsong |
| |
| Institution: | Yan Guangwen, Yin Hongmei, Liu Changsong (1. Talimu University, A La Er 843300; 2. Sichuan Agricultural University, Yaan 625014) |
| |
| Abstract: | Aspiration and laceration were used to collected oocytes in the study. The average number of A grade oocytes collected by aspiration per ovary was significantly lower than that of laceration, but the average number of B and C grade of oocytes collected by aspiration were more than that of laceration. The maturing rate of oocyte collected from ovaries without corepus luteum were 84.33%, which is higher than that of the ovaries with eorepus luteum（74.32%） significantly. In this study, the maturing rate of both surface follicular oocytes and IO-oocytes were more than 75%. The cleavage rate of surface follicular oocytes is 54.76%, which is higher than that of IO-oocytes with unremarkablly. The ideal cultivation system could be obtained by adding cumulus oophorus cells in culture fluid in IVM period and adding uterine tubal epithelium cells in IVC period. |
| |
| Keywords: | Bovine Oocyte IVM IVC IO-oocyte |
| 本文献已被 维普 万方数据 等数据库收录！ |
