| 不同植物生长调节物质对南瓜组织培养及植株再生的影响 |
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| 引用本文： | 邹克琴,张拥军,楼纪东,何颖,庞一飞.不同植物生长调节物质对南瓜组织培养及植株再生的影响[J].安徽农业科学,2008,36(13):5311-5313. |
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| 作者姓名： | 邹克琴 张拥军 楼纪东 何颖 庞一飞 |
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| 作者单位： | 中国计量学院生命科学学院,浙江杭州,310018;中国计量学院生命科学学院,浙江杭州,310018;中国计量学院生命科学学院,浙江杭州,310018;中国计量学院生命科学学院,浙江杭州,310018;中国计量学院生命科学学院,浙江杭州,310018 |
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| 摘 要： | 目的]为南瓜属植物优良品种的快速繁育、抗逆性品种的选育等提供研究基础。方法]以"锦粟2号"南瓜叶片为外植体,用不同植物生长调节物质进行组织培养和植株再生。结果]结果表明:试验条件下,诱导叶片分化形成愈伤组织的最佳培养基为MS+6-BA1.0 mg/L+NAA 0.1 mg/L;最适的芽分化培养基为MS+6-BA 1.0 mg/L+NAA 0.5 mg/L;诱导芽生根的最佳培养基为1/2 MS+NAA 0.5mg/L,芽生根率为100%。结论]成功获得了"锦粟2号"南瓜的愈伤组织及再生植株。
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| 关 键 词： | 南瓜 组织培养 植株再生 |
Effect of Different Plant Growth Regulators on Tissue Culture and Plantlet Regeneration of Pumpkin |
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| ZOU Ke-qin et al.Effect of Different Plant Growth Regulators on Tissue Culture and Plantlet Regeneration of Pumpkin[J].Journal of Anhui Agricultural Sciences,2008,36(13):5311-5313. |
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| Authors: | ZOU Ke-qin |
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| Abstract: | Objective] The research aimed to provide foundation for the rapid propagation of superior variety and the selection of resistant variety of Cucurbita.Method] The leaves of "Jinsu 2" was used as the explant and treated with different plant growth regulators in tissue culture and plantlet regeneration.Results] The best culture for leaves callus inducement was MS 6-BA 1.0 mg/L NAA 0.1 mg/L.The most suitable culture for bud rooting inducement was 1/2 MS NAA 0.5 mg/L,and the percentage of rooting was 100%.Conclusion] Tissue culture and plantlet regeneration of "Jinsu 2" was obtained successfully. |
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| Keywords: | Pumpkin Tissue culture Plantlet regeneration |
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