| 发酵床中耐高温地衣芽孢的分离鉴定及产酶分析 |
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| 引用本文： | 尹红梅,吴迎奔,张德元,王震,陈薇,贺月林.发酵床中耐高温地衣芽孢的分离鉴定及产酶分析[J].家畜生态学报,2012,33(6):97-102. |
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| 作者姓名： | 尹红梅 吴迎奔 张德元 王震 陈薇 贺月林 |
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| 作者单位： | 湖南省微生物研究所 |
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| 基金项目： | 湖南省科技厅重大专项(2009FJ 1005);湖南省生猪产业技术体系生猪产业规模养殖与环境控制岗位项目资助 |
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| 摘 要： | 从健康猪生长的发酵床垫料中筛选到1株耐高温的高效降解菌Y34,把其接种到猪粪水中反应25 d后，对COD与NH3 N降解率分别为84.7%，83.5%。生长特性表明其最适生长温度为50 ℃。通过形态观察、生理生化鉴定及16S rDNA序列比对，鉴定菌株Y34为地衣芽孢杆菌（Bacillus licheniformis），其与Bacillus licheniformis相似性为99.0%。对此菌发酵过程中产酶情况作了研究，结果表明，在50℃培养条件下，菌株Y34能够分泌过氧化氢酶、蛋白酶、脲酶，具有用于研制发酵床微生物制剂的潜力。
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| 关 键 词： | 发酵床 地衣芽孢杆菌 鉴定 酶活 |
| 收稿时间： | 2012-08-08 |
Isolation and Identification of Thermophilic Bacillus licheniformis Strain in Fermentation-bed and Its Properties of Heat-Adapted Enzymes Production |
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| YIN Hong-mei,WU Ying-ben,ZHANG De-yuan,WANG Zhen,CHEN Wei,HE Yue-lin.Isolation and Identification of Thermophilic Bacillus licheniformis Strain in Fermentation-bed and Its Properties of Heat-Adapted Enzymes Production[J].Acta Ecologae Animalis Domastici,2012,33(6):97-102. |
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| Authors: | YIN Hong-mei WU Ying-ben ZHANG De-yuan WANG Zhen CHEN Wei HE Yue-lin |
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| Institution: | (Hunan Institute of Microbiology,Changsha,Hunan 410009,China) |
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| Abstract: | Bacterial strain Y34 ，isolated from fermentation bed materials of healthy growing pigs， was capable of high efficient degradation and heat adapted performance. The results showed that，in livestock wastewater, after 25 days of degradation，the removal rates of COD and NH3 N were 84.7% and 83.5% by Y34.The optimal temperature for the growth of Y34 was 50 ℃.Based on morphological，physiological，biochemical characteristics，and 16S rDNA phylogenetic analysis, strain Y34 was identified as Bacillus licheniformis．High similarities of 16S rDNA sequence with Bacillus licheniformis was 99.0%.The GenBank accession number is JN848790. It was found that this strain could secrete catalase, protease and urease during the culture at 50℃ and thus was a good candidate for microbial agent used in fermentation bed. |
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| Keywords: | fermentation bed Bacillus licheniformis identification enzyme activities |
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