| 用苹果酸通透酶基因的特异性引物进行酒酒球菌快速鉴定 |
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| 引用本文： | 刘延林,蒋思欣,李 华.用苹果酸通透酶基因的特异性引物进行酒酒球菌快速鉴定[J].西北农林科技大学学报(社会科学版),2006,34(9):193-196. |
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| 作者姓名： | 刘延林 蒋思欣 李 华 |
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| 作者单位： | 1. 西北农林科技大学,葡萄酒学院,陕西,杨凌,712100
2. 中国科学院,微生物研究所,北京,100080 |
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| 基金项目： | 陕西省自然科学基金;西北农林科技大学校科研和教改项目 |
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| 摘 要： | 以Oenococcus oeni苹果酸通透酶基因为目标基因,设计了1对特异性引物PmlepL/PmlepR进行酒酒球菌的快速鉴定研究。结果表明,直接以O.oeni的菌落为模板,通过PmlepL/PmlepR引物的PCR扩增,可得到苹果酸通透酶基因的特异性条带;用此特异性引物进行供试乳酸菌的PCR鉴定,所有O.oeni菌系均得到特异性条带,而供试的其他种类乳酸菌未扩增出目标带。引物PmlepL/PmlepR可用于O.oeni的快速PCR鉴定。
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| 关 键 词： | 苹果酸通透酶基因 特异引物 快速鉴定 酒酒球菌 |
| 文章编号： | 1671-9387(2006)09-0193-04 |
| 收稿时间： | 2005-10-19 |
| 修稿时间： | 2005年10月19 |
Design and evaluation of specific primers targeted mleP gene for rapid identification of Oenococcus oeni |
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| LIU Yan-lin,JIANG Si-xin,LI Hua.Design and evaluation of specific primers targeted mleP gene for rapid identification of Oenococcus oeni[J].Journal of Northwest Sci-Tech Univ of Agr and,2006,34(9):193-196. |
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| Authors: | LIU Yan-lin JIANG Si-xin LI Hua |
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| Institution: | 1 (1 College of Enology,Northwest A & F University,Yangling,Shaanxi 712100,China; 2 Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China) |
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| Abstract: | Specific primers of malate permease gene(mleP) targeted were designed and evaluated for the rapid identification of Oenococcus oeni.The result indicated that specific stripes of mleP gene could be cloned by PCR from colonies of O.oeni directly.Meanwhile,the specific stripes of mleP gene could be cloned by PCR from all the strains of O.oeni but failed from several other species of lactic acid bacteria.The PCR assay confirmed to be specific for O.oeni species and can be used for rapid identification of O.oeni. |
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| Keywords: | malate permease gene specific primer rapid identification Oenococcus oeni |
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