| 与亚洲棉光籽突变体DPL972中光籽基因紧密连锁的SSR分子标记的开发与应用 |
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| 引用本文： | 冯晓旭,邹长松,陆才瑞,程海亮,张友平,Mkulama A P Mtawa,王巧连,宋国立.与亚洲棉光籽突变体DPL972中光籽基因紧密连锁的SSR分子标记的开发与应用[J].棉花学报,2016,28(4):392-398. |
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| 作者姓名： | 冯晓旭 邹长松 陆才瑞 程海亮 张友平 Mkulama A P Mtawa 王巧连 宋国立 |
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| 作者单位： | 中国农业科学院棉花研究所/棉花生物学国家重点实验室,河南安阳,455000 |
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| 基金项目： | The National Natural Science Foundation of China(31301369);National High-Tech R&D Program(863 Program)(2013AA102601) |
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| 摘 要： | 对棉纤维相关性状基因的克隆及遗传分析可为阐明纤维形成机制奠定良好基础.以野生型材料DPL971及其光籽突变体DPL972为亲本构建F2群体,其中光籽与毛籽性状分离比符合3∶1,表明突变体DPL972中的光籽基因为显性单基因(将其命名为GaFzl).结合棉花基因组数据,合成1567对SSR(Simple sequence repeat)引物,覆盖了A组13条染色体,标记多态性分析和群体连锁分析发现在染色体A08上存在15对与光籽基因GaFzl连锁的标记,其中最近的标记为SSR82,遗传距离为6.6 cM.本实验完成了GaFzl基因的染色体初步定位,开发了15个与其连锁的SSR标记,为下一步图位克隆奠定了基础.
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| 关 键 词： | 纤维 光籽突变体 GaFzl SSR 连锁图 |
Simple Sequence Repeat Markers Closely Linked with a New Fuzzless Gene in Fuzzless Mutant DPL972 (Gossypium arboreum) |
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| Feng Xiaoxu;Zou Changsong;Lu Cairui;Cheng Hailiang;Zhang Youping;Mkulama A P Mtawa;Wang Qiaolian;Song Guoli.Simple Sequence Repeat Markers Closely Linked with a New Fuzzless Gene in Fuzzless Mutant DPL972 (Gossypium arboreum)[J].Cotton Science,2016,28(4):392-398. |
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| Authors: | Feng Xiaoxu;Zou Changsong;Lu Cairui;Cheng Hailiang;Zhang Youping;Mkulama A P Mtawa;Wang Qiaolian;Song Guoli |
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| Institution: | Institute of Cotton Research of Chinese Academy of Agricultural Sciences / State Key Laboratory of Cotton Biology, Anyang, Henan 455000, China |
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| Abstract: | The genetic analysis and cloning of genes associated with cotton fiber characteristics will not only reveal the mechanism of fiber development, but will also aid molecular breeding. The cotton (Gossypium arboretum) mutant DPL972 is fuzzless, providing a crucial germplasm resource for cotton research. An F2 population derived from a cross between the wild-type DPL971 and the mutant DPL972 was constructed, and the results of trait investigation fitted a segregation ratio of 3∶1, which indicated that the fuzzless trait was controlled by a single dominant gene (named here as GaFzl). A linkage map was constructed, which included 15 simple sequence repeat markers close to the candidate target gene. The fuzzless gene GaFzl was inferred to be located on chromosome A08 and was tightly linked with marker SSR82 at a genetic interval of 6.6 cM. |
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| Keywords: | fiber fuzzless mutant GaFzl SSR linkage map |
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