| 血皮槭叶绿体DNA非编码区差异序列筛选和分析 |
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| 引用本文： | 叶学敏,于雪丹,付其迪,郑勇奇,张川红.血皮槭叶绿体DNA非编码区差异序列筛选和分析[J].林业科学研究,2017,30(4):674-678. |
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| 作者姓名： | 叶学敏 于雪丹 付其迪 郑勇奇 张川红 |
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| 作者单位： | 中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091,中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091,中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091,中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091,中国林业科学研究院林业研究所, 国家林业局林木培育重点实验室, 北京 100091 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金（CAFYBB2016MA001） |
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| 摘 要： | 目的]筛选出高变异率的叶绿体DNA序列,以进行血皮槭天然群体的谱系地理学研究。方法]以血皮槭16个天然群体为试材,对叶绿体基因组20个非编码区序列进行测序研究,并利用筛选出的高变异率cpDNA序列初步分析了其遗传变异。结果]序列比对和系统发育分析结果显示血皮槭cpDNA种内变异非常低,9个cpDNA序列检测到不同程度的变异位点,其中只有4个序列psbJ-petA、ndhF-rpl32、trnD-trnT和trnH-psbA表现出较高的变异水平。结论]筛选出的4个高变异率cpDNA序列,可以在分子水平上研究血皮槭种内的系统发育、遗传变异和种群历史动态,为进一步研究濒危种血皮槭的分子谱系地理学奠定基础。
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| 关 键 词： | 血皮槭 cpDNA 序列筛选 序列比对 系统发育分析 |
| 收稿时间： | 2016/11/3 0:00:00 |
Differential Analysis of Non-coding Chloroplast DNA Sequences in Acer griseum |
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| YE Xue-min,YU Xue-dan,FU Qi-di,ZHENG Yong-qi and ZHANG Chuan-hong.Differential Analysis of Non-coding Chloroplast DNA Sequences in Acer griseum[J].Forest Research,2017,30(4):674-678. |
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| Authors: | YE Xue-min YU Xue-dan FU Qi-di ZHENG Yong-qi and ZHANG Chuan-hong |
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| Institution: | Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China,Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China,Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China,Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China and Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
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| Abstract: | Objective] By screen highly variable cpDNA sequences to study the phylogeography of Acer griseum. Method] Twenty non-coding cpDNA sequences were sequenced. The genetic variation of sixteen natural populations in A. griseum was preliminarily analyzed with the selected highly variable sequences. Result] Sequences alignment and phylogenetic analysis showed that the intraspecific variation of cpDNA in A. griseum was very low. Different variation were detected in some loci of nine cpDNA sequences and a relative high level of variation displayed in four sequences psbJ-petA, ndhF-rpl32, trnD-trnT and trnH-psbA. Conclusion] The four selected highly variable cpDNA sequences could be used to study the phylogeny and genetic variation and clarify the history of population dynamics of A. griseum. These sequences will lay a foundation for future phylogeographic study in A. griseum. |
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| Keywords: | Acer griseum cpDNA sequences screening sequences alignment phylogenetic analysis |
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