| 狂犬病病毒糖/核融合基因原核表达克隆质粒的构建与分析 |
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| 引用本文： | 姜海龙,钱爱东.狂犬病病毒糖/核融合基因原核表达克隆质粒的构建与分析[J].吉林农业大学学报,2005,27(6):683-686. |
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| 作者姓名： | 姜海龙 钱爱东 |
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| 作者单位： | 吉林农业大学动物科技学院,长春,130118 |
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| 基金项目： | 国家自然科学基金资助项目（30070542） |
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| 摘 要： | 试验将编码狂犬病病毒的糖蛋白和核蛋白基因经PCR扩增后，通过限制性核酸内切酶风PstⅠ的酶切并以T4 DNA连接酶进行连接。将所获得的基因片断与pMD-18T载体连接。经转化到大肠杆菌JM109和质粒大量制备后，进行酶切和测序鉴定。结果表明：PCR扩增片段与文献报道的狂犬病糖蛋白和核蛋白核酸序列没有差异，所构建的克隆质粒正确，新构建的融合基因没有出现新的抗原位点。
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| 关 键 词： | 狂犬病病毒 糖蛋白 核蛋白 |
| 文章编号： | 1000-5684（2005）06-0683-04 |
| 收稿时间： | 2005-03-20 |
| 修稿时间： | 2005-03-20 |
Construction and Analysis of Prokaryotic Expression Plasmid Containing Fusion Gene of Glycoprotein/Neucleoprotein of Rabies Virus |
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| JIANG Hai-long,QIAN Ai-dong.Construction and Analysis of Prokaryotic Expression Plasmid Containing Fusion Gene of Glycoprotein/Neucleoprotein of Rabies Virus[J].Journal of Jilin Agricultural University,2005,27(6):683-686. |
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| Authors: | JIANG Hai-long QIAN Ai-dong |
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| Institution: | College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China |
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| Abstract: | The experiment was conducted to construct the prokaryotic expression plasmid which contained the fusion rabies virus gene encoding the Glycoprotein/Neucleoprotein after PCR amplification of G gene and N gene of Rabies virus. The Pst I was used to digest the two target genes and linked the two genes with T4DNA ligase. The fusion gene was cloned into pMD-18T vector, the enzyme digesting the plasmid was identified and the sequences were analyzed. The results indicated that the construction of expression vector containing the fusion gene was correct and no new antigen site was found in the fusion gene. |
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| Keywords: | Rabies virus glycoprotein neucleoprotein |
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