| 抗菌肽MB39基因导入‘皇家嘎啦’苹果及其四倍体植株的培育 |
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| 引用本文： | 刘庆忠,赵红军,刘鹏,Mong Rengong.抗菌肽MB39基因导入‘皇家嘎啦’苹果及其四倍体植株的培育[J].园艺学报,2001,28(5):392-395. |
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| 作者姓名： | 刘庆忠 赵红军 刘鹏 Mong Rengong |
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| 作者单位： | (山东省果树研究所, 泰安271000 ; USDA/ ARS HCRL , Corvallis , OR 97330 , USA; 3USDA/ ARS , Fruit Laboratory , Beltsville , MD 20705 , USA) |
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| 基金项目： | 山东省科委良种产业化工程资助项目;山东省人事厅资助项目;山东省农业科学院资助项目(98-06) |
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| 摘 要： | 采用农杆菌介导法, 将抗菌肽MB39基因转入‘皇家嘎啦’苹果, 获得了7 个转基因株系。通过秋水仙素诱变染色体组工程育种技术, 由转化体叶片获得了20 个四倍体植株。经PCR 检测和Southern Blotting 杂交证明, 抗菌肽MB39基因已经整合到皇家嘎啦苹果的染色体组中。转基因株系TR21、TR23 提高了对火疫病的抗性。采用流式细胞技术( Flow Cytometry) 确定植株为四倍体。
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| 关 键 词： | 抗菌肽MB39 转基因 苹果 四倍体 秋水仙素 |
| 文章编号： | 0513-353(2001)05-0392-07 |
| 修稿时间： | 2000年11月6日 |
Regeneration of Tetraploid Plants with Cecropin MB39 Gene from ' Royal Gala' Apple |
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| Liu Qingzhong ,Zhao Hongjun ,Liu Peng ,Mong Rengong ,and Freddi A.Hammerschlag.Regeneration of Tetraploid Plants with Cecropin MB39 Gene from '''' Royal Gala'''' Apple[J].Acta Horticulturae Sinica,2001,28(5):392-395. |
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| Authors: | Liu Qingzhong Zhao Hongjun Liu Peng Mong Rengong and Freddi AHammerschlag |
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| Institution: | 1. Department of Neurology, The First Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China;
2. Experimental Room of Physiology Science, Sun Yat-sen University of Medical Sciences, Guangzhou 510080, China |
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| Abstract: | As part of the program to develop transgenic Malus ×Domestic. cv. ‘Royal Gala’tetraploid with improved traits , new germplasmof transgenic diploid and tetraploid plants with cecropin MB39 gene were regenerated. Seven transgenic diploid plants were obtained from etiolated internodalexplants by Agrobacterium tumefaciens-mediated transformation using the plasmid binary vector pGV containing a chimeric gene cecropin MB39 . The integration of the cecropin gene into apple genome was confirmed by PCR and Southern blotting analysis. It was proved that lines , TR-1 and TR-3 , had increased the resistance to fireblight disease caused by Erwinia amylovora. Twenty tetraploid lines were produced by cocultivating leaf explants from wild type and transgenic diploid shoots with colchicine in apple regeneration medium. Flow cytometry was used for ploidy etermination. The tetraploid plants were distinguishable from the diploid on morphological as well as cytogenetic grounds. Both the transgenic diploid and tetraploid plants are now being evaluated for growth development and disease resistance. |
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| Keywords: | Cecropin MB 39 Transgenic Apple Tetraploid Colchicine |
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