| 元江普通野生稻金属硫蛋白(YJMT-2)的获得及序列分析 |
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| 引用本文： | 史冬燕.元江普通野生稻金属硫蛋白(YJMT-2)的获得及序列分析[J].四川农业大学学报,2009,27(1). |
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| 作者姓名： | 史冬燕 |
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| 作者单位： | 山东菏泽学院,生命科学系,山东,菏泽,274015;云南省农业科学院,生物技术与种质资源研究所,云南,昆明,650223 |
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| 基金项目： | 国家自然科学基金，云南省应用基础研究重点项目，云南省科技攻关项目 |
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| 摘 要： | 对SMARTTM技术构建的元江普通野生稻叶片cDNA文库克隆进行随机测序,获得了元江普通野生稻金属硫蛋白(YJMT-2)的cDNA序列。该序列全长为558 bp,5′-端非翻译区为84 bp,3′-端非翻译区为247 bp,开放阅读框长度为225 bp(包括一个终止密码子),可编码74个氨基酸,蛋白分子量为7.51 kD,理论等电点(PI)为6.51,其中含12个半胱氨酸(Cys),占了总氨基酸的16.22%,且集中分布在肽链的N端和C端,呈CXC排列方式,具有植物MT-2的典型特征;经Pfam15.0分析证明,其属于金属硫蛋白家族的成员。BlastP同源性分析,该基因与日本粳稻、大麦的同源性较高,分别为98%和65%。首次得到元江普通野生稻金属硫蛋白基因,为其功能研究提供了基础。
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| 关 键 词： | 元江普通野生稻 金属硫蛋白 cDNA 序列分析 |
Cloning and Sequence Analysis of Metallothionein (YJMT-2) from Yuanjiang O. rifupogon |
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| SHI Dong-Yan.Cloning and Sequence Analysis of Metallothionein (YJMT-2) from Yuanjiang O. rifupogon[J].Journal of Sichuan Agricultural University,2009,27(1). |
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| Authors: | SHI Dong-Yan |
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| Abstract: | After sequencing Yuanjiang O.rifupogon's cDNA library constructed by SMARTTM technology,the cDNA of metallothionein(YJMT-2)was cloned.It was 558 bp,including 84 bp of 5′ untranslated region and 247 bp of 3′ untranslated region;the open reading frame(ORF) was 225 bp,encoding 74 amino acids.The protein molecular weight was 7.51 kD and theoretical isoelectric point(PI) was 6.51,including 12 cysteines,which accounted for 16.22% of total of content amino acids,and distributed at peptidechain in N terminal and C terminal;they presented CXC alignment and were characterized by MT-2 of plant.YJMT-2 gene analyzed by Pfam 15.0 was a member of metallothi onein family.Amino acids sequence of YJMT-2 gene with BlastP shared the highest similarity with Oryza sativa Japonica and Hordeum vulgare,which had homology of 98% and 65%,respectively the first time that metallothionein genes were obtained from Yuanjiang O.rifupogon.It provides a good basis for further functional study. |
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| Keywords: | cDNA |
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