| 狼尾草属牧草rDNA的ITS序列分析 |
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| 引用本文： | 陈志彤,黄勤楼,潘伟彬,黄毅斌.狼尾草属牧草rDNA的ITS序列分析[J].草业学报,2010,19(4):135-141. |
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| 作者姓名： | 陈志彤 黄勤楼 潘伟彬 黄毅斌 |
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| 作者单位： | 1.福建农林大学生命科学学院,福建 福州350002;
2.福建省农业科学院农业生态研究所 福建省山地草业工程技术
研究中心,福建 福州350013;
3.闽西职业技术学院资源工程系,福建 龙岩364021 |
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| 基金项目： | 福建省科技厅重点项目;国家科技支撑计划 |
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| 摘 要： | 对来自福建、江苏、海南等15份狼尾草属牧草的5.8SrDNA、ITS1及ITS2片段进行克隆及序列分析,并登录于GenBank数据库,采用DNAMAN、CLUSTALX、MEGA等软件分析其遗传关系聚类图。结果显示,克隆的目的片段长度为573～586bp,聚类结果总体能较好地反应狼尾草属牧草之间的遗传距离,其中杂交狼尾草和细茎杂交狼尾草可能存在着同种异名的现象。
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| 关 键 词： | 狼尾草 ITS 牧草 |
| 收稿时间： | 2009-06-22 |
Sequence analysis of the rDNA ITS region of Pennisetum species (Poaceae) |
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| CHEN Zhi-tong,HUANG Qin-lou,PAN Wei-bin,HUANG Yi-bin.Sequence analysis of the rDNA ITS region of Pennisetum species (Poaceae)[J].Acta Prataculturae Sinica,2010,19(4):135-141. |
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| Authors: | CHEN Zhi-tong HUANG Qin-lou PAN Wei-bin HUANG Yi-bin |
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| Institution: | 1.College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2.Agricultural Ecology Research Institute, Fujian Academy of Agricultural Sciences/Fujian ;
Engineering and Technology Research Center for Hilly Prataculture, Fuzhou 350013, ;
China;
3.Minxi Vocational and Technical College, Resource Engineering ;
Department, Longyan 364021, China |
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| Abstract: | The internal transcribed spacer (ITS) regions of nuclear ribosomal DNA from 15 species of Pennisetum (Poaceae) from areas such as Fujian, Jiangsu, Hainan were sequenced and the sequences submitted to the Genbank database. The phylogenetic relationship of the 15 species were studied by CLUSTAL X and MEGA software. Their length of nrDNA ITS region ranged from 573 to 586 bp. P. americanum×P. purpureum and P. americanum×P. purpureum cv. slimstem may be the same variety with different names. The phylogram tree indicated that sequence analysis of nrDNA ITS regions is a good technique for studying the genetic diversity of Pennisetum. |
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