| 茄子果实中α-茄碱提取方法的研究 |
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| 引用本文： | 周宝利,李志文,丁昱文,刘翔,叶雪凌.茄子果实中α-茄碱提取方法的研究[J].园艺学报,2009,36(1):141-146. |
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| 作者姓名： | 周宝利 李志文 丁昱文 刘翔 叶雪凌 |
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| 作者单位： | (沈阳农业大学园艺学院，沈阳110161；天津大学农业与生物工程学院，天津 300072) |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以茄子品种'辽茄七号'果实为试验材料，设计了单因素试验和正交试验，采用高效液相色谱法对不同提取方式、不同超声波提取条件下茄子果皮和果肉中α-茄碱的含量进行测定。结果表明：采用超声波提取法时，茄子果实中α-茄碱的提取量最高，提取前后α-茄碱性质保持不变。优化的超声波提取条件为：以料液比为1:10的70%甲醇为提取剂，在50 ℃下提取60 min。测得果皮中α-茄碱平均提取量为0.107 mg·g-1，相对标准偏差 (RSD) 为1.80%（n=5），果肉中α-茄碱平均提取量为0.620 mg·g-1，RSD为1.29%（n=5），平均回收率为98.0%，RSD为1.23%（n=3），说明该方法的提取率和精密度高、稳定性好，合理可行。
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| 关 键 词： | 茄子 果实 α-茄碱 超声波提取 |
| 收稿时间： | 2008-5-26 |
| 修稿时间： | 2008-12-15 |
Extraction Method of a-solanine in Eggplant Fruit |
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| ZHOU Bao-li,LI Zhi-wen,DING Yu-wen,LIU Xiang,YE Xue-ling.Extraction Method of a-solanine in Eggplant Fruit[J].Acta Horticulturae Sinica,2009,36(1):141-146. |
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| Authors: | ZHOU Bao-li LI Zhi-wen DING Yu-wen LIU Xiang YE Xue-ling |
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| Institution: | (College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China; College of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China) |
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| Abstract: | Single-factor and orthogonal experiments were conducted to establish a rapid and efficient extraction method of α-solanine from eggplant peel and sarcocarp of eggplant fruit. Different extraction methods were evaluated for their efficiency. The content of α-solanine in the extracts was determined by HPLC. Results showed that the most efficient extraction was obtained when ultrasonic wave was used. Parameters affecting the extraction efficiency of the ultrasonic wave were further optimized. The optimized procedure was to mix the smashed material with 70% methanol (1:10, V/V) and then to apply ultrasonic-wave to the mixture for 60 min at 50 ℃. With the use of the optimized method, the average content of α-solanine extracted from peel and sarcocarp was mg·g-1 and 0.620 mg·g-1 with RSD (relative standard deviation) = 1.80% (n=5) and 1.29% (n=5), respectively, and the average recovery rate was 98.0% with a RSD of 1.23% (n=3). Result analysis indicated that the extraction method established in our study was good in extraction rate, accuracy and reproducibility and was applicable in the extraction of α-solanine from eggplant fruit. |
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| Keywords: | eggplant fruit α-solanine ultrasonic wave extraction |
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