| 内生真菌对菊花幼苗干旱胁迫生理的影响 |
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| 引用本文： | 刘晓珍,宋文玲,张凯,叶宇成,戴传超.内生真菌对菊花幼苗干旱胁迫生理的影响[J].园艺学报,2011,38(2):335. |
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| 作者姓名： | 刘晓珍 宋文玲 张凯 叶宇成 戴传超 |
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| 作者单位： | （江苏省微生物资源产业化工程技术研究中心，江苏省微生物与功能基因组学重点实验室，南京师范大学生命科学学院，南京 210046） |
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| 基金项目： | 国家自然科学基金项目，江苏省滩涂生物资源与环境保护重点实验室开放项目 |
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| 摘 要： | 比较了内生葡萄孢菌（C1）和球毛壳菌（C4）菌株诱导下的菊花苗和对照菊花苗，经30 % PEG6000胁迫不同天数后，叶片超氧化物歧化酶（SOD）、过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）活性，叶片丙二醛（MDA）、可溶性蛋白含量，根系活力，以及胁迫15 d后菊花的总生物量。结果表明：PEG胁迫15 d后，C1、C4处理和对照的生物量分别为4.1、4.4和3.3 g，差异达到显著水平。对照根系活力、PAL活性在胁迫前期迅速变化，接菌处理在胁迫前期均基本稳定，延缓了胁迫反应，但随着胁迫时间的延长，各处理差异不显著； MDA含量接菌处理3 d后达到峰值，随后稳定而对照则继续增加，6 d时达峰值，随后逐渐降低，胁迫后期接菌处理和对照无显著差异；各处理POD活性、可溶性蛋白含量都随着胁迫时间的延长先增加后降低，但各处理之间差异不显著。短期干旱条件下，内生真菌有利于菊花耐旱性的提高。
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| 关 键 词： | 菊花 PEG6000 葡萄孢菌 球毛壳菌 抗旱性 抗氧化 |
| 收稿时间： | 2010-09-03 |
Effects of Two Kinds of Endophytic Fungi Infection on Water Stress of Seedlings of Chrysanthemum morifolium |
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| LIU Xiao-zhen,SONG Wen-ling,Zhang Kai,Ye Yu-cheng,DAI Chuan-chao.Effects of Two Kinds of Endophytic Fungi Infection on Water Stress of Seedlings of Chrysanthemum morifolium[J].Acta Horticulturae Sinica,2011,38(2):335. |
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| Authors: | LIU Xiao-zhen SONG Wen-ling Zhang Kai Ye Yu-cheng DAI Chuan-chao |
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| Institution: | （Jiangsu Engineering and Technology Research Center for Industrialization of Microbial Resources，Jiangsu Key Laboratory for microbes and functional genomics，College of Life Science，Nanjing Normal University，Nanjing 210046，China） |
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| Abstract: | The water stress was simulated to study the effect of the endophytic fungi Botrytis sp.（C1）or Chaetomium globosum（C4）on Chrysanthemum morifolium in the adverse circumstance. C. morifolium plantlets which were inoculated with C1，C4 and cultured 60 days in the pots were stressed by 30% PEG6000 solution. SOD activity，POD activity，PAL activity，root activity，MDA content and soluble protein content were determined at 0，3，6，9，12，15 days after treatments，and the biomass of C. morifolium were also determined at 15 days after treatment. The results showed that the biomass of C1，C4 group and the control reached 4.1，4.4 and 3.3 g respectively，and all treatments got significant deviation（P < 0.05）. With PEG treatment，the root activity and PAL activity of the control were more sensitive at the first period of PEG stress，while fungi treatment groups were stabled，and stress reactions were delayed by fungi treatment. MDA content of the two fungi treatments reached the peak values with PEG stress for 3 days，while the control continues increased and got the peak at 6 days with PEG treatment. When at the later period of the stress，root activity，PAL activity，and MDA content of all treatments had no significant deviation. POD activity and soluble protein content of all treatments were increased firstly and decreased then，but all treatments had no siginificant deviation. Endophytic fungi can increase the drought resistance of C. morifolium in a short period of water stress. |
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| Keywords: | PEG6000 |
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