| 草鱼→鲤的细胞核移植 |
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| 引用本文： | 齐福印,许桂珍,郑明霞.草鱼→鲤的细胞核移植[J].中国兽医学报,1992(1). |
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| 作者姓名： | 齐福印 许桂珍 郑明霞 |
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| 作者单位： | 内蒙古畜牧学院
(齐福印，许桂珍)，内蒙古医学院(郑明霞) |
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| 摘 要： | 用草鱼的头肾组织细胞核、草鱼的头肾细胞核与鲤鱼成熟去核卵之杂种胚胎细胞核、草鱼的囊胚细胞核,分别与鲤鱼的去核卵细胞质配合进行核移植操作.结果,体细胞核移植,在亚科间的组合——草鱼核 鲤质中获得原肠期胚胎,得率为1%;体细胞继代核移植,在草鱼核 鲤质中获得胚孔封闭期胚胎,得率为5%;胚胎细胞核移植,在草鱼核 鲤质中获得眼球色素出现期胚胎,得率为2%.草鱼→鲤核-质杂种胚胎的胎盘形状、大小与鲤鱼的相似,而大于草鱼;胚盘细胞数量也多于草鱼胚胎同期胚盘,而类似于鲤鱼同期胚盘.
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| 关 键 词： | 草鱼 鲤鱼 细胞核移植 |
The Nuclear Transplantation of Grass Carp (Ctenopharyngodon idellus Cuvier et Valenciennes) and Cyprinoid Fish (Cyprinus car- pio Linnaeus) |
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| Qi Fuyin et al.The Nuclear Transplantation of Grass Carp (Ctenopharyngodon idellus Cuvier et Valenciennes) and Cyprinoid Fish (Cyprinus car- pio Linnaeus)[J].Chinese Journal of Veterinary Science,1992(1). |
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| Authors: | Qi Fuyin |
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| Institution: | The Inner Mongolian Animal Husbandry College |
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| Abstract: | This paper reported the nuclear transplantation between 2 species of fish belonging to different subfamilies with distant kinship, the grass carp and cyprinoid fish. The experimental results were as follows: In the combination of grass carp nucleus cyprinoid fish cytoplasm, embryos at the archenteron stage were obtained, the yield rate was 1%. The nuclear transplantation of second generation showed that in combination of grass carp nucleus cypri-noid fish cytoplasm, 5% of embryos at germinal-aperture-sealing stage were obtained. In the transplantation of embryonic nucleus, grass carp nucleus cyprinoid fish cytoplasm combination obtained 2% of embryos at the eyeball-pigment-appearing stage. Based on the observations of the characteristics of the germinal diskes and their cell numbers, it is suggested that the nucleo-cyto-plasmic hybrid was created from grass carp and cyprinoid fish. |
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| Keywords: | grass carp (Ctenopharyngodon idellus) cyprinoid fish (Cyprinus carpio) nuclear transplantation |
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