| 荔枝FLOWERING LOCUST(FT)同源基因cDNA全长克隆及其表达 |
| |
| 引用本文： | 丁峰,彭宏祥,何新华,李冬波,朱建华,秦献泉,李鸿莉,罗聪,曹辉庆.荔枝FLOWERING LOCUST(FT)同源基因cDNA全长克隆及其表达[J].果树学报,2012(1):75-80,160. |
| |
| 作者姓名： | 丁峰 彭宏祥 何新华 李冬波 朱建华 秦献泉 李鸿莉 罗聪 曹辉庆 |
| |
| 作者单位： | 广西大学农学院;广西农业科学院园艺研究所;广西作物遗传改良与生物技术重点实验室 |
| |
| 基金项目： | 国家荔枝龙眼产业技术体系熟期育种岗位(CARS-33-03);农业部热带作物种质资源保护专项(11RZZY-35) |
| |
| 摘 要： | 应用RT-PCR方法在首次克隆获得荔枝AP1同源基因cDNA全长基础上,又得到2个荔枝FT同源基因cDNA全长,分别命名为LcFT1和LcFT2(基因登录号分别为:JN214350、JN214351)。LcFT1基因开放阅读框522 bp,编码174个氨基酸,推测蛋白质分子质量为19.65 ku,等电点为8.68。LcFT2基因开放阅读框522 bp,编码174个氨基酸,推测蛋白质分子质量为19.56 ku,等电点为7.34。蛋白质二级结构预测表明,LcFT1和LcFT2蛋白都具有4个α螺旋,10个β折叠区。同源分析表明,LcFT1和LcFT2基因在不同植物中的一致性为72%～82%。半定量RT-PCR分析表明,三月红荔枝花芽分化期LcFT1和LcFT2基因只在叶中表达,并且在成熟叶中表达量最多。研究将有助于进一步了解荔枝开花的分子机理及其成花的生物学发育过程。
|
| 关 键 词： | 荔枝 FLOWERING LOCUST(FT)基因 序列分析 表达模式 |
Cloning and expression analysis of the FLOWERING LOCUS T(FT) homologous gene cDNA from Litchi chinensis |
| |
| DING Feng,PENG Hong-xiang,HE Xin-hua,LI Dong-bo,ZHU Jian-hua,QIN Xian-quan,LI Hong-li,LUO Cong,CAO Hui-qing.Cloning and expression analysis of the FLOWERING LOCUS T(FT) homologous gene cDNA from Litchi chinensis[J].Journal of Fruit Science,2012(1):75-80,160. |
| |
| Authors: | DING Feng PENG Hong-xiang HE Xin-hua LI Dong-bo ZHU Jian-hua QIN Xian-quan LI Hong-li LUO Cong CAO Hui-qing |
| |
| Institution: | 1College of Agriculture,Guangxi University,Nanning,Guangxi 530004 China;2Horticulture Research Institute,Guangxi Academy of Agricultural Sciences,Nanning,Guangxi 530007 China;3Guangxi Crop Genetic Improvement and Biotechnology Lab,Guangxi,Nanning 530007 China) |
| |
| Abstract: | Two full-length cDNA sequences of homologous FT genes were cloned based on homologous AP1 gene we had firstly cloned by employing RT-PCR from Litchi chinensis,which were named as LcFT1 and LcFT2(GenBank accession No.JN214350,JN214351).The open reading frame of LcFT1 gene is 522 bp in length,encoding a protein of 174 amino acids,with an estimated molecular weight and an isoelectric point of 19.65 ku and 8.68 respectively.The open reading frame of LcFT2 gene is 522 bp in length,encoding a protein of 174 amino acids,with an estimated molecular weight and an isoelectric point of 19.56 ku and 7.34 respectively.Prediction of the secondary structure of the protein showed that LcFT1 and LcFT2 proteins all have 4 α helices and 10 β sheets.A comparison of the nucleotide sequences of homologous FT genes from different species indicated that LcFT1,LcFT2 genes have a range of 72% to 82% identity in nucleotide sequence with homologues of other plants.Expression analysis by RT-PCR indicted that LcFT1 and LcFT2 genes expressed only in leaf during flower bud differentiation period of Sanyuehong Litchi chinensis but expressed much in mature leaf.The research is beneficial to the further understanding of the molecular mechanism of Litch flowering and biological developmental stages of flowering. |
| |
| Keywords: | Litchi chinensis FLOWERING LOCUS T(FT) gene Sequence analysis Expression pattern |
| 本文献已被 CNKI 等数据库收录！ |
|
