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超声波处理对大白菜花粉管通道法转基因的影响
引用本文:狄红梅,王学府,曹秋芬,孟玉平.超声波处理对大白菜花粉管通道法转基因的影响[J].农业科学与技术,2011(12):1773-1776.
作者姓名:狄红梅  王学府  曹秋芬  孟玉平
作者单位:[1]晋中职业技术学院,山西榆次030600 [2]山西省农业生物技术研究中心,山西太原030031
基金项目:山西省科技攻关项目(021034);山西省农科院科研项目(YGX-01).
摘    要:目的]探索提高花粉管通道法对大白菜转化率的途径。方法]以大白菜品种03号和玉青的花粉为材料,对其萌发的缓冲液和超声波处理的各参数进行筛选。结果]大白菜花粉萌发的最佳缓冲液为蔗糖200g/L+硼酸100mg/L+硝酸钙200mg/L,较理想的超声波处理参数为功率150W、工作时间5s、间隔时间5s、处理次数8s;经浓度为200mg/L的卡那霉素筛选,获得3株T1代单株。结论]为进一步进行大白菜的基因转化奠定了基础。

关 键 词:超声波  大白菜  花粉管通道法  转基因

Effects of Ultrasonication on Genetic Transformation via the Pollen-Tube Pathway in Chinese Cabbage
DI Hong-mei,WANG Xue-fu,CAO Qiu-fen,MENG Yu-ping.Effects of Ultrasonication on Genetic Transformation via the Pollen-Tube Pathway in Chinese Cabbage[J].Agricultural Science & Technology,2011(12):1773-1776.
Authors:DI Hong-mei  WANG Xue-fu  CAO Qiu-fen  MENG Yu-ping
Institution:1.Jinzhong Vocational and Technical College,Yuci 030600;2.Agricultural Biotechnology Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031 )
Abstract:Objective] This research aimed to explore the method to increase conversion rate of pollen-tub pathway in Chinese cabbage.Method] Chinese cabbage varieties Yuqing and No.03 were used as materials for the selection of germination buffer and parameters for ultrasonication.Result] The optimal buffer for pollen germination of Chinese cabbage was 200 g/L sucrose + 100 mg/L boric acid + 200 mg/L calcium nitrate,the preferred ultrasonic processing power was 150 W,processing time was 5 s,interval time was 5 s and processing frequency was 8.Three T1-generation plants were obtained through selection with 200 mg/L kanamycin.Conclusion] This research laid foundation for the further genetic transformation of Chinese cabbage.
Keywords:Ultrasonication  Chinese cabbage  Pollen-tub pathway  Genetic transformation
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