| 超声波处理对大白菜花粉管通道法转基因的影响 |
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| 引用本文： | 狄红梅,王学府,曹秋芬,孟玉平.超声波处理对大白菜花粉管通道法转基因的影响[J].农业科学与技术,2011(12):1773-1776. |
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| 作者姓名： | 狄红梅 王学府 曹秋芬 孟玉平 |
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| 作者单位： | [1]晋中职业技术学院,山西榆次030600 [2]山西省农业生物技术研究中心,山西太原030031 |
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| 基金项目： | 山西省科技攻关项目（021034）;山西省农科院科研项目（YGX-01）. |
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| 摘 要： | 目的]探索提高花粉管通道法对大白菜转化率的途径。方法]以大白菜品种03号和玉青的花粉为材料,对其萌发的缓冲液和超声波处理的各参数进行筛选。结果]大白菜花粉萌发的最佳缓冲液为蔗糖200g/L＋硼酸100mg/L＋硝酸钙200mg/L,较理想的超声波处理参数为功率150W、工作时间5s、间隔时间5s、处理次数8s;经浓度为200mg/L的卡那霉素筛选,获得3株T1代单株。结论]为进一步进行大白菜的基因转化奠定了基础。
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| 关 键 词： | 超声波 大白菜 花粉管通道法 转基因 |
Effects of Ultrasonication on Genetic Transformation via the Pollen-Tube Pathway in Chinese Cabbage |
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| DI Hong-mei,WANG Xue-fu,CAO Qiu-fen,MENG Yu-ping.Effects of Ultrasonication on Genetic Transformation via the Pollen-Tube Pathway in Chinese Cabbage[J].Agricultural Science ＆ Technology,2011(12):1773-1776. |
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| Authors: | DI Hong-mei WANG Xue-fu CAO Qiu-fen MENG Yu-ping |
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| Institution: | 1.Jinzhong Vocational and Technical College,Yuci 030600;2.Agricultural Biotechnology Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031 ) |
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| Abstract: | Objective] This research aimed to explore the method to increase conversion rate of pollen-tub pathway in Chinese cabbage.Method] Chinese cabbage varieties Yuqing and No.03 were used as materials for the selection of germination buffer and parameters for ultrasonication.Result] The optimal buffer for pollen germination of Chinese cabbage was 200 g/L sucrose ＋ 100 mg/L boric acid ＋ 200 mg/L calcium nitrate,the preferred ultrasonic processing power was 150 W,processing time was 5 s,interval time was 5 s and processing frequency was 8.Three T1-generation plants were obtained through selection with 200 mg/L kanamycin.Conclusion] This research laid foundation for the further genetic transformation of Chinese cabbage. |
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| Keywords: | Ultrasonication Chinese cabbage Pollen-tub pathway Genetic transformation |
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