| 一株高抗铬真菌的分离筛选及其特性的研究 |
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| 引用本文： | 赖洁玲,唐晓燕,钟礼松,林谦,吴弦华,韦明肯.一株高抗铬真菌的分离筛选及其特性的研究[J].农业科学与技术,2011(12):1765-1768. |
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| 作者姓名： | 赖洁玲 唐晓燕 钟礼松 林谦 吴弦华 韦明肯 |
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| 作者单位： | 玉林师范学院生命科学与技术学院,广西玉林537000 |
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| 基金项目： | 国家自然科学基金（30960008）;广西教育厅项目（200810LX393）;广西玉林师范学院高层次人才启动项目;广西玉林师范学院专项研究项目（2011YJZX01）;广西玉林师范学院青年科研项目（2010YJQN24）. |
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| 摘 要： | 目的]从受铬污染的土壤中分离抗铬微生物。方法]利用微生物分离纯化技术,在培养基中加入不同浓度的Cr^6＋,经过不断的分离和驯化筛选耐铬真菌,根据其形态特征和菌落特征进行初步鉴定,并对其生物学特性进行了初步研究,包括生长曲线的测定、温度、pH值和渗透压对其生长的影响。结果]从玉林市周边地区受重金属污染较严重的10处土壤中分离筛选到1株在Cr（VI）浓度为1000mg/L时仍生长良好的抗铬真菌,根据其形态特征和菌落特征初步鉴定该菌为酵母菌,该菌在15～37℃的温度范围内生长良好,最适温度为28℃;pH在4～10时,菌株均能生长良好,其中最适生长pH为7.2;在NaCl浓度为0.5%～5%时,均能生长良好。通过抗性试验,发现该菌除了对铬具有较高的抗性外,对其它的重金属Pb、Cu、Fe和复合重金属Pb＋Cu、Cu＋Fe、Pb＋Fe、Pb＋Cu＋Fe也具有抗性能力。结论]该试验筛选到的抗铬真菌对自然环境具有较好的适应性,同时该菌对其他重金属也具有抗性。
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| 关 键 词： | 抗Cr（Ⅵ）真菌 分离筛选 生物学特征 |
Study on Screening of Fungi with High-resistance against Chromium and on Related Characteristics |
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| LAI Jie-ling,TANG Xiao-yan,ZHONG Li-song,LIN Qian,WU Xian-hua,WEI Ming-ken.Study on Screening of Fungi with High-resistance against Chromium and on Related Characteristics[J].Agricultural Science ＆ Technology,2011(12):1765-1768. |
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| Authors: | LAI Jie-ling TANG Xiao-yan ZHONG Li-song LIN Qian WU Xian-hua WEI Ming-ken |
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| Institution: | (School of Life Science and Technology,Yulin Normal University,Yulin 537000 ) |
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| Abstract: | Objective] The aim was to separate chromium-resistant microorganism from soil contaminated by chromium.Method] Separation and purification technique was used as follows：different concentrations of Cr^6＋ were added into medium,and chromium-resistant fungi were screened after separations and domestications.The selected fungi were under preliminary identification according to its morphological and colony characteristics.Then,related biological characteristics were studied,including measurement of growth curve,growing effects by temperature,pH value and osmotic pressure.Result] The Cr（VI） with concentration of 1 000 mg/L was separated and selected from soils in ten different places contaminated seriously by heavy metal in adjacent region of Yulin City.Considering its morphological and colony characteristics,it was preliminarily identified as saccharomycetes,which can well grow within 15-37 ℃,and whose most suitable temperature was 28℃.Bacterial strain can grow well with pH of 4-10,and the optimum pH was 7.2;besides,it can grow well with NaCl concentration of 0.5%-5.0%.Through the experiment,the bacteria was found with resistance not only to chromium,but also to heavy metals such as Pb＋Cu,Cu＋Fe,Pb＋Fe,and Pb＋Cu＋Fe.Conclusion] The fungi selected from the experiment were of good adaptability to natural environment,and it also had resistance to other heavy metals. |
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| Keywords: | Bcteria with resistance to Cr（Ⅵ） Separation and selection Biological characteristics |
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